Strain Data Sheet
RBRC10968
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10968 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6(Cg)-Sox1<em1(cre)Okn> |
| Former Common name | Triple lineagelabelling mice (Sox12A-Cre Delta Puro) |
| H-2 Haplotype | |
| ES Cell line | B6NJ-22<UTR> [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background. |
| Strain description | Knock-in mice of Cre at the C-terminus of protein coding sequence of the Sox1 gene. |
| Colony maintenance | |
| References | Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers. Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H Development, 146(21):dev174938 (2019). 31597657 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2023/11/27Room:4-BRack:DSentinel mouse program 2023/08/28Room:4-BRack:DSentinel mouse program 2023/05/29Room:4-BRack:DSentinel mouse program 2023/04/10Room:4-BRack:DAdditional tests |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 8 | Frt | ||||
| P2A | porcine teschovirus-1 2A peptide | 8 | P2A | ||||
| Sox1 MGI:98357 | SRY (sex determining region Y)-box 1 | 8 | Sox1<em1(cre)Okn> | endonuclease-induced mutation 1, Hideyuki Okano | |||
| cre | Phage P1 Cre recombinase | 8 | cre | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | 8 | nls | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | P2A (porcine teschovirus-1 2A peptide), SV40 virus NLS, bacteriophage P1 Cre, Saccharomyces cerevisia FRT site, mouse Sox1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 146(21). pii: dev174938 (2019). |
| Depositor | Hideyuki Okano (Keio University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |