Strain Data Sheet

RBRC10969

Strain Information

Image
BRC No.RBRC10969
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-T<em1(dre)Okn>
Former Common nameTriple lineagelabelling mice (brachyury2A-Dre Delta Puro)
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background.
Strain descriptionKnock-in mice of Dre at the C-terminus of protein coding sequence of the T gene.
Colony maintenance
References
Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers.
Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H
Development, 146(21):dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2024/11/25Room:4-BRack:HSentinel mouse program
2024/08/26Room:4-BRack:HSentinel mouse program
2024/05/27Room:4-BRack:HSentinel mouse program
2024/02/26Room:4-BRack:HSentinel mouse program
2023/11/27Room:4-BRack:HSentinel mouse program
2023/08/30Room:4-BRack:HAdditional tests
2023/08/28Room:4-BRack:HSentinel mouse program
2023/05/29Room:4-BRack:HSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dre Bacteriophage D6 recombinase17Dre
F3 yeast FRT (flippase recombination target) site (synthetic) variant17F3
P2A porcine teschovirus-1 2A peptide17P2A
T
MGI:98472		brachyury, T-box transcription factor T17T<em1(dre)Okn> endonuclease-induced mutation 1, Hideyuki Okano
  • neural tube defect(MedGEN)

  • sacral agenesis-abnormal ossification of the vertebral bodies-persistent notochordal canal syndrome(MedGEN)
    • nls SV40 large T antigen nuclear localization signal (NLS)17nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAmouse T/brachyury genomic DNA, P2A (porcine teschovirus-1 2A peptide), P1-like transducing phage D6 Dre, SV40 virus NLS, Saccharomyces cerevisia FRT variant(F3)
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 146(21). pii: dev174938 (2019).
    DepositorHideyuki Okano (Keio University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data