Strain Data Sheet

RBRC10970

Strain Information

Image
BRC No.RBRC10970
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-Sox17<em1(phiC31<*>)Okn>
Former Common nameTriple lineagelabelling mice (Sox17 2A-phiC31o Delta Puro)
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background.
Strain descriptionKnock-in mice of phiC31o at the C-terminus of protein coding sequence of the Sox17 gene.
Colony maintenance
References
Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers.
Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H
Development, 146(21):dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2023/08/28Room:4-BRack:DSentinel mouse program
2023/05/29Room:4-BRack:DSentinel mouse program
2023/04/10Room:4-BRack:DAdditional tests

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
F5 yeast FRT (flippase recombination target) site (synthetic) variant1F5
P2A porcine teschovirus-1 2A peptide1P2A
PhiC31o PhiC31 recombinase1PhiC31o rat tyrosine hydroxylase (TH) promoter
Sox17
MGI:107543		SRY (sex determining region Y)-box 171Sox17<em1(phiC31<*>)Okn> endonuclease-induced mutation 1, Hideyuki Okano
  • vesicoureteral reflux 3(MedGEN)
    • nls SV40 large T antigen nuclear localization signal (NLS)1nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP2A (porcine teschovirus-1 2A peptide), SV40 virus NLS, Streptomyces lividans phiC31o, Saccharomyces cerevisia FRT site(F5), mouse Sox17 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2019 Nov 4;146(21). pii: dev174938
    DepositorHideyuki Okano (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data