Strain Data Sheet

RBRC10972

Strain Information

Image
BRC No.RBRC10972
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Serpinh1<tm2Kzn>
Former Common nameHsp47 flox
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Kazuhiro Nagata, Kyoto University in 2012. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. C57BL/6 congenic strain by backcrossing for 7 generations.
Strain descriptionSerpinh1 (Hsp47) floxed mice. A loxP site was inserted upstream of exon 6. An FRT-flanked neo cassette with a 3'loxP site was inserted downstream of exon 6.
Colony maintenance
References
The molecular chaperone Hsp47 is essential for cartilage and endochondral bone formation.
Masago Y, Hosoya A, Kawasaki K, Kawano S, Nasu A, Toguchida J, Fujita K, Nakamura H, Kondoh G, Nagata K
J. Cell Sci., 125(Pt 5):1118-28 (2012). 22492985

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site7Frt
Frt yeast FRT (flippase recombination target) site7Frt
Serpinh1
MGI:88283		serine (or cysteine) peptidase inhibitor, clade H, member 17Serpinh1<tm2Kzn>
MGI:5505265		targeted mutation 2, Kazuhiro Nagata
  • osteogenesis imperfecta type 10(DisGeNET, MedGEN)

  • preterm premature rupture of the membranes(MedGEN)
    • loxP phage P1 loxP7loxP
    loxP phage P1 loxP7loxP
    neo neomycin resistance gene (E. coli)7 mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAbacteriophage P1 loxP sites, yeast FRT (flippase recombination target) sites, Mouse Phosphoglycerate kinase promoter(PGK promoter), synthetic bacterial EM7 promoter (T7 phage), E. coli Neomycin resistant gene, mouse Serpinh1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 125(Pt 5):1118-28 (2012). The RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorKazuhiro Nagata (Kyoto Sangyo University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Shin J, Toyoda S, Okuno Y, Hayashi R, Nishitani S, Onodera T, Sakamoto H, Ito S, Kobayashi S, Nagao H, Kita S, Otsuki M, Fukuhara A, Nagata K, Shimomura I.
    HSP47 levels determine the degree of body adiposity.
    Nat Commun 14(1) 7319(2023) 37951979