Strain Information | |
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Image | |
BRC No. | RBRC10977 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6JJcl.Cg-Mdga1<tm1.1Tymo> |
Former Common name | Mdga1 KO |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Tohru Yamamoto, Faculty of Pharmaceutical Science, Hokkaido University. TT2 (B6CBF1 derived) ES cells were used to generate Mdga1 knockout mice. The mutant mice were backcrossed to C57BL/6J. C57BL/6J congenic strain. |
Strain description | Mdga1 gene knockout mice. Exon 1 of the Mdga1 gene was replaced with a lacZ and frt flanked neo cassette (neo cassette was removed by FLPe-mediated recombination). Homozygous mutant mice exhibit defective migration and positioning of neurons during corticogenesis. |
Colony maintenance | |
References | Brain Res. 2006 Jul 26;1101(1):12-9. Epub 2006 Jun 19. 16782075 Cell Rep. 2017 Dec 26;21(13):3637-3645 29281813 Am. J. Med. Genet. B Neuropsychiatr. Genet., 2008 Oct 5;147B(7):1089-100. 18384059 Dev. Dyn., 240(1):96-107 (2011). 21104742 Neurosci Lett., 716:134677 (2020). 31812551 Schizophr Res. 2011 Feb;125(2-3):194-200 21146959 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Mdga1MAM domain containing glycosylphosphatidylinositol anchor 117Mdga1<tm1.1Tymo>targeted mutation 1.1, Toru Yamamoto Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site17Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin 3' end17HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)17 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox71mutant lox sequence17lox71 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP |
Ordering Information | |
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Donor DNA | P1 phage lox71 site, E.coli LacZ, rabbit beta-globin 3’end, Yeast frt site, P1 phage loxP site, mouse PGK 3’end, mouse Mdga1 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0454K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Dyn. 2011 240(1):96-107.) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | Tohru Yamamoto (Kagawa University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |