Strain Data Sheet
RBRC10980
Strain Information | |
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| Image | |
| BRC No. | RBRC10980 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6NJcl.Cg-Mdga2<tm1Tymo> |
| Former Common name | Mdga2 KO |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Tohru Yamamoto, Faculty of Pharmaceutical Science, Hokkaido University. TT2 (B6CBF1 derived) ES cells were used to generate Mdga2 knockout mice. The mutant mice were backcrossed to C57BL/6N. C57BL/N congenic strain. |
| Strain description | Mdga2 gene knockout mice. Exon 1 of the Mdga2 gene was replaced with lacZ and neo cassette. |
| Colony maintenance | |
| References | Genome-wide analyses of exonic copy number variants in a family-based study point to novel autism susceptibility genes. Bucan M, Abrahams B S, Wang K, Glessner J T, Herman E I, Sonnenblick L I, Alvarez Retuerto A I, Imielinski M, Hadley D, Bradfield J P, Kim C, Gidaya N B, Lindquist I, Hutman T, Sigman M, Kustanovich V, Lajonchere C M, Singleton A, Kim J, Wassink T H, McMahon W M, Owley T, Sweeney J A, Coon H, Nurnberger J I, Li M, Cantor R M, Minshew N J, Sutcliffe J S, Cook E H, Dawson G, Buxbaum J D, Grant S F, Schellenberg G D, Geschwind D H, Hakonarson H PLoS Genet. 2009 Jun;5(6):e1000536. 19557195Altered Cortical Dynamics and Cognitive Function upon Haploinsufficiency of the Autism-Linked Excitatory Synaptic Suppressor MDGA2. Connor S A, Ammendrup-Johnsen I, Chan A W, Kishimoto Y, Murayama C, Kurihara N, Tada A, Ge Y, Lu H, Yan R, LeDue J M, Matsumoto H, Kiyonari H, Kirino Y, Matsuzaki F, Suzuki T, Murphy T H, Wang Y T, Yamamoto T, Craig A M Neuron. 2016 Sep 7;91(5):1052-1068 27608760 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 12 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 12 | Frt | ||||
| Mdga2 MGI:2444706 | MAM domain containing glycosylphosphatidylinositol anchor 2 | 12 | Mdga2<tm1Tymo> | targeted mutation 1, Toru Yamamoto | |||
| lacZ | beta-galactosidase (E. coli) | 12 | |||||
| lox71 | mutant lox sequence | 12 | lox71 | ||||
| loxP | phage P1 loxP | 12 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 12 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | P1 phage lox71 site, E.coli LacZ, rabbit beta-globin 3’end, Yeast frt sites, mouse PGK promoter, E.coli neo, P1 phage loxP site, mouse PGK 3’end, mouse Mdga2 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0704K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Neuron. 91(5):1052-68.) Please inform theDEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
| Depositor | Tohru Yamamoto (Kagawa University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |