Strain Data Sheet

RBRC10983

Strain Information

Image
BRC No.RBRC10983
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hcn4<tm1(tTA)Nnka> Hcn4<tm1.1(Luc/EGFP)Nna>
Former Common nameHCN4 tet/luc mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Noriyuki Nakashima and Makoto Takano, Kurume University in 2017.
Strain descriptionThis strain has two different alleles at the Hcn4 gene locus. One is a reporter allele in which luciferase/EGFP fusion gene was inserted in frame of translation initiation codon of the Hcn4 gene. Hcn4 gene is disrupted. The other is an allele in which tTA/Tre cassette was inserted at the translation initiation site of the Hcn4 gene.
Colony maintenance
References
HCN4 pacemaker channels attenuate the parasympathetic response and stabilize the spontaneous firing of the sinoatrial node.
Kozasa Y, Nakashima N, Ito M, Ishikawa T, Kimoto H, Ushijima K, Makita N, Takano M
J. Physiol., 596(5):809-825 (2018). 29315578

Health Report

Examination Date / Room / Rack2023/03/13Room:3-3Rack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)9EGFP
GH human growth hormone gene9GH
Hcn4
MGI:1298209		hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1(tTA)Nnka>
MGI:5560747		targeted mutation 1, Noriyuki Nakashima
  • Brugada syndrome 8(MedGEN)

  • sick sinus syndrome 2, autosomal dominant(MedGEN)
    • Hcn4
    MGI:1298209    		hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1.1(Luc/EGFP)Nna> targeted mutation 1.1, Noriyuki Nakashima
  • Brugada syndrome 8(MedGEN)

  • sick sinus syndrome 2, autosomal dominant(MedGEN)
    • URA3 Orotidine-5'-phosphate (OMP) decarboxylase (yeast)9URA3
    loxP phage P1 loxP9loxP
    loxP phage P1 loxP9loxP
    loxP phage P1 loxP9loxP
    luc luciferase9luc
    neo neomycin resistance gene (E. coli)9 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)9tTA
    SV40 polyA signal9
    SV40 polyA signal9

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAfirefly Luciferase cDNA, jellyfish EGFP cDNA, Simian virus SV40, P1 phage loxP, Human human growth hormone gene, cytomegalovirus cytomegalovirus promoter, herpes simplex virus HSV thymidine kinase polyadenylation signal、HSV thymidine kinase promoter, E coli tetracycline transactivator、tet operator、neomycin resistant gene、yeast URA3 gene
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 596(5):809-825 (2018).For use of the BIOLOGICAL RESOURCE by a for-profit institution the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorMakoto Takano (Kurume University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data