Strain Information | |
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Image | |
BRC No. | RBRC10984 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | B6;B6D2-Tg(CAG-hCas9)2Saku |
Former Common name | sCAT (systemically Cas9-expressing Transgenic mice) |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Devekoped by Takayuki Sakurai, Shinshu University in 2013. A TG construct was injected into eggs derived from B6D2FlxB6J. Mixed genetic background. |
Strain description | Transgenic mice expressing hCas9 under the control of CAG promoter. |
Colony maintenance | |
References | A non-inheritable maternal Cas9-based multiple-gene editing system in mice. Sakurai T, Kamiyoshi A, Kawate H, Mori C, Watanabe S, Tanaka M, Uetake R, Sato M, Shindo T Sci. Rep., 6:20011 (2016). 26817415Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9. Sakurai T, Kamiyoshi A, Kawate H, Watanabe S, Sato M, Shindo T Sci. Rep., 10(1):1091 (2020). 31974430 |
Health Report | |
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Examination Date / Room / Rack | 2024/12/09Room:3-3Rack:BSentinel mouse program 2024/09/09Room:3-3Rack:BSentinel mouse program 2024/06/10Room:3-3Rack:BSentinel mouse program 2024/03/11Room:3-3Rack:BSentinel mouse program 2023/12/11Room:3-3Rack:BSentinel mouse program 2023/09/11Room:3-3Rack:BSentinel mouse program 2023/06/13Room:3-3Rack:BSentinel mouse program 2023/03/13Room:3-3Rack:BSentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Cas9 | CRISPR associated protein 9 | UN | Cas9 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
FLAG | FLAG tag (synthetic) | UN | FLAG |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Synthetic Flag tag, Streptococcus pyogenes Cas9 cDNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 6:20011 (2016). Sci. Rep., 10(1):1091 (2020). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE b a for-profit institution the RECIPIENT must reach a agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Takayuki Sakurai (Shinshu University) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month May 2021 |
BRC mice in Publications |
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No Data |