Strain Information | |
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Image | |
BRC No. | RBRC10984 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6;B6D2-Tg(CAG-hCas9)2Saku |
Former Common name | sCAT (systemically Cas9-expressing Transgenic mice) |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Devekoped by Takayuki Sakurai, Shinshu University in 2013. A TG construct was injected into eggs derived from B6D2FlxB6J. Mixed genetic background. |
Strain description | Transgenic mice expressing hCas9 under the control of CAG promoter. |
Colony maintenance | |
References | Sci. Rep., 10(1):1091 (2020). 31974430 Sci. Rep., 6:20011 (2016). 26817415 |
Health Report | |
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Examination Date / Room / Rack | 2024/03/11Room:3-3Rack:B 2023/12/11Room:3-3Rack:B 2023/09/11Room:3-3Rack:B 2023/06/13Room:3-3Rack:B 2023/03/13Room:3-3Rack:B 2022/12/12Room:3-3Rack:B 2022/09/12Room:3-3Rack:B 2022/06/13Room:3-3Rack:B 2022/05/16Room:3-3Rack:B |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cas9CRISPR associated protein 9UNCas9CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAG |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Synthetic Flag tag, Streptococcus pyogenes Cas9 cDNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2016 Jan 28;6:20011, Sci Rep. 2020; 10: 1091.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE b a for-profit institution the RECIPIENT must reach a agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Takayuki Sakurai (Shinshu University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |