Strain Data Sheet
RBRC10992
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10992 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6.Cg-Tg(Prnp-TARDBP*R208)#Koya |
| Former Common name | PrP-TDP43 R208 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed and deposited by Dr. Koji Yamanaka. Tg mice were generated using BDF1 and backcrossed to C57BL/6J for more than 10 generations. |
| Strain description | Transgenic mice expressing mutated human TARDBP (R208) protein with 3x FLAG tag at C-terminal under the control of mouse Prnp promoter. |
| Colony maintenance | |
| References | TDP-43 accelerates age-dependent degeneration of interneurons. Tsuiji H, Inoue I, Takeuchi M, Furuya A, Yamakage Y, Watanabe S, Koike M, Hattori M, Yamanaka K Sci. Rep., 7(1):14972 (2017). 29097807 |
Health Report | |
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| Examination Date / Room / Rack | 2023/03/13Room:3-5Rack:DSentinel mouse program |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| FLAG | 3xFLAG tag (synthetic) | UN | FLAG | ||||
| TARDBP | TAR DNA binding protein (human) | UN | TARDBP | mouse prion promoter | |||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse prion genomic DNA, human TDP-43 gene and mutant(TDP-43-R208), FLAG tag |
| Research application | Neurobiology Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 7(1):14972 (2017). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. Otherwise, the availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must understand the intellectual property of the MoPrP vector is owned by Johns Hopkins University. Therefore, an acknowledgment for use of the MoPrP vector to Johns Hopkins University is requested. |
| Depositor | Koji Yamanaka (Nagoya University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |