Strain Data Sheet
RBRC11094
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11094 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | C57BL/6J-Ddx4<em1(cre/ERT2)Utr> |
| Former Common name | B6-Ddx4<em1(CreERT2)Utr> |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Seiya Mizuno, University of Tuskuba in 2020. This strain was generated with the CRISPR/Cas9. C57BL/6J background. |
| Strain description | Cre/ERT2 knock-in mice at the C-terminal portion of the Ddx4 gene via 2A peptide. Homozygous males are infertile. |
| Colony maintenance | |
| References | Generation of B6-Ddx4(em1(CreERT2)Utr) , a novel CreERT2 knock-in line, for germ cell lineage by CRISPR/Cas9. Le H T, Hasegawa Y, Daitoku Y, Kato K, Miznuo-Iijima S, Dinh T T H, Kuba Y, Osawa Y, Mikami N, Morimoto K, Ayabe S, Tanimoto Y, Murata K, Yagami K I, Takahashi S, Mizuno S, Sugiyama F Genesis, 58(7):e23367 (2020). 32293787 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/11/18Room:4-ARack:KSentinel mouse program 2024/08/19Room:4-ARack:KSentinel mouse program 2024/05/20Room:4-ARack:KSentinel mouse program 2024/02/19Room:4-ARack:KSentinel mouse program 2023/11/20Room:4-ARack:KSentinel mouse program 2023/08/21Room:4-ARack:KSentinel mouse program 2023/05/22Room:4-ARack:KSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Ddx4 MGI:102670 | DEAD box helicase 4 | 13 | Ddx4<em1(cre/ERT2)Utr> | endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center | |||
| ERT2 | estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A) | 13 | ERT2 | ||||
| HBB2 | rabbit(Oryctolagus cuniculus) beta globin polyA | 13 | HBB2 | ||||
| P2A | porcine teschovirus-1 2A peptide | 13 | P2A | ||||
| cre | Phage P1 Cre recombinase | 13 | cre | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | 13 | nls | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | P2A (porcine teschovirus-1 2A peptide), Simian virus 40 nuclear localization signal (NLS), bacteriophage P1 Cre recombinase, human (Homo sapiens)estrogen receptor 1 (alpha) cDNA, rabbit(Oryctolagus cuniculus) beta globin polyA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 58(7):e23367 (2020). |
| Depositor | Seiya Mizuno (University of Tsukuba) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Oct 2022 |
BRC mice in Publications |
|---|
| No Data |