Strain Information | |
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Image | |
BRC No. | RBRC11098 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Scx<tm1.1(EGFP)Skom> |
Former Common name | Scx-EGFP |
H-2 Haplotype | |
ES Cell line | V6.5 [(C57BL/6J x 129S4/SvJae)F1] |
Background strain | |
Appearance | |
Strain development | Deposited by Shingo Komura, Gifu University in 2020. Mice were generated using v6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1. Mice were further backcrossed to C57BL/6J for 6-7 generations. C57BL/6J congenic line. |
Strain description | Mice harboring IRES EGFP knock in at 3’ UTR of the Scx gene. |
Colony maintenance | |
References | Sci. Rep., 10(1):3992 (2020). 32132649 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)15EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA15HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)15 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site15rox |
Ordering Information | |
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Donor DNA | Encephalomyocarditisvirus (EMCV) internal ribosomal entry site (ires), Aequorea Victoria EGFP cDNA, Rabbit beta globin pA, D6 Bacteriophage rox site, mouse Scx genomic DNA |
Research application | Cell Biology Research Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2020 Mar 4;10(1):3992.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Shingo Komura (Gifu University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |