Strain Data Sheet

RBRC11130

Strain Information

Image
BRC No.RBRC11130
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tubb3<tm1.1(Cre)Ksak>
Former Common nameTubb3-Cre
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Kenji Sakimura, Niigata University in 2020. Mice were generated using RENKA ES cells derived from C57BL/6N. C57BL/6N genetic background.
Strain descriptionCre/IRES/EGFP knockin mice targeted at the Tubb3 gene locus.
Colony maintenance
References
Optical manipulation of local cerebral blood flow in the deep brain of freely moving mice.
Abe Y, Kwon S, Oishi M, Unekawa M, Takata N, Seki F, Koyama R, Abe M, Sakimura K, Masamoto K, Tomita Y, Okano H, Mushiake H, Tanaka K F
Cell Rep., 36(4):109427(2021). 34320360

Health Report

Examination Date / Room / Rack2024/12/09Room:3-5Rack:BSentinel mouse program
2024/08/19Room:3-5Rack:BSentinel mouse program
2024/06/10Room:3-5Rack:BSentinel mouse program
2024/03/11Room:3-5Rack:BSentinel mouse program
2023/12/11Room:3-5Rack:BSentinel mouse program
2023/09/13Room:3-5Rack:BAdditional tests
2023/09/11Room:3-5Rack:BSentinel mouse program
2023/06/13Room:3-5Rack:BSentinel mouse program
2023/03/13Room:3-5Rack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Green Fluorescent Protein (Aequorea victoria)8EGFP
Frt yeast FRT (flippase recombination target) site8Frt
Ires internal ribosomal entry site (EMCV)8
Tubb3
MGI:107813		tubulin, beta 3 class III8Tubb3
  • complex cortical dysplasia with other brain malformations 1(MedGEN)

  • fibrosis of extraocular muscles, congenital, 3A, with or without extraocular involvement(DisGeNET, MedGEN)
    • cre Phage P1 Cre recombinase8cre
    nls SV40 large T antigen nuclear localization signal (NLS)8nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNASV40 large T antigen nuclear localization signal (NLS), Bacteriophage P1 Cre recombinase, EMCV internal ribosomal entry site (IRES), Aequorea victoria EGFP cDNA, Saccharomyces cerevisiae Frt site, mouse Tubb3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 36(4):109427(2021).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorKenji Sakimura (Niigata University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data