Strain Data Sheet
RBRC11140
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11140 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Dnase1l3<tm1Dkit> Dffb<tm1Osa> |
| Former Common name | CAD-/-DNase1L3-/- double knockout mouse |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Daisuke Kitamura, Tokyo University of Science in 2020. |
| Strain description | Double mutant of the Dnase1l3 and Dffb. |
| Colony maintenance | |
| References | Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation. Kawane K, Fukuyama H, Yoshida H, Nagase H, Ohsawa Y, Uchiyama Y, Okada K, Iida T, Nagata S Nat. Immunol., 4, 138-144 (2003). 12524536DNase gamma is the effector endonuclease for internucleosomal DNA fragmentation in necrosis. Mizuta R, Araki S, Furukawa M, Furukawa Y, Ebara S, Shiokawa D, Hayashi K, Tanuma S, Kitamura D PLoS One, 8(12):e80223 (2013). 24312463Cell-free DNA in blood circulation is generated by DNase1L3 and caspase-activated DNase. Watanabe T, Takada S, Mizuta R Biochem. Biophys. Res. Commun., 516, 790-795 (2019). 31255286 |
Health Report | |
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| Examination Date / Room / Rack | 2024/12/09Room:3-2Rack:ASentinel mouse program 2024/09/09Room:3-2Rack:ASentinel mouse program 2024/06/10Room:3-2Rack:ASentinel mouse program 2024/03/11Room:3-2Rack:ASentinel mouse program 2023/12/11Room:3-2Rack:ASentinel mouse program 2023/09/11Room:3-2Rack:ASentinel mouse program 2023/06/26Room:3-2Rack:AAdditional tests 2023/06/13Room:3-2Rack:ASentinel mouse program 2023/03/13Room:3-2Rack:ASentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Dnase1l3 MGI:1314633 | deoxyribonuclease 1-like 3 | 14 | Dnase1l3<tm1Dkit> MGI:5581508 | targeted mutation 1, Daisuke Kitamura | |||
| loxP | phage P1 loxP | 14 | loxP | ||||
| loxP | phage P1 loxP | 14 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 14 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
| Dffb MGI:1196287 | DNA fragmentation factor, beta subunit | 4 | Dffb<tm1Osa> MGI:2449797 | targeted mutation 1, Shigekazu Nagata | |||
| neo | neomycin resistance gene (E. coli) | 4 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | P1 phage loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistant gene, Mouse Pgk1 PolyA signal, Mouse DNase1l3 genomic DNA, Herpes simplex virus Thymidine kinase gene promoter, Mouse Dffb genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 516, 790-795 (2019). Nat. Immunol., 4, 138-144 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Daisuke Kitamura (Tokyo University of Science) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |