Strain Data Sheet

RBRC11168

Strain Information

Image
BRC No.RBRC11168
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Itgae<tm1.1Ksat>
Former Common nameCd103<-/-> mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Katsuaki Sato, RIKEN in 2020. Mice were generated using JN/2 ES cells derived from C57BL/6. C57BL/6 genetic background.
Strain descriptionItgae mutant mice. STOP cassette was kncoked-in at the exon 1 of the Cd103 (Itgae).
Colony maintenance
References
Pivotal role of CD103 in the development of psoriasiform dermatitis.
Fukui T, Fukaya T, Uto T, Takagi H, Nasu J, Miyanaga N, Nishikawa Y, Koseki H, Choijookhuu N, Hishikawa Y, Yamashita Y, Sato K
Sci. Rep., 10(1):8371 (2020). 32433498

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)11EGFP
Frt yeast FRT (flippase recombination target) site11Frt
HBEGF heparin-binding EGF-like growth factor (human)11HBEGF
IRES2 internal ribosomal entry site (EMCV)11IRES2
Itgae
MGI:1298377		integrin alpha E, epithelial-associated11Itgae<tm1.1Ksat> targeted mutation 1.1, Katsuaki Sato
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
SV40 polyA11

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNASaccharomyces cerevisiae FRT site, human HB-EGF cDNA, EMCV IRES2, Aequorea Victoria EGFP cDNA, Simian virus 40 polyA, Saccharomyces cerevisiae DEAD-box ATP-dependent RNA helicase DED1, Phage P1 loxP sites, mouse Itgae genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. May 20;10(1):8371 (2020). doi: 10.1038/s41598-020-65355-9.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep. 10:8371, 2020. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Dr. Katsuaki Sato) using the Approval Form. The Recipient acknowledges that RIKEN (and/or its original provider) reserves title to the Material. The Recipient will not transfer the Material to any scientists. The Recipient will not publish or present Research result without obtaining prior permission fromthe DEVELOPER (Dr. Katsuaki Sato). The Recipient will send Dr. Katsuaki Sato any abstract of submission, oral or written presentation, and/or copy of draft of paper or research article which is resulted from the Research at least 30 days prior to the submission. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEVELOPER (Dr. Katsuaki Sato).
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data