Strain Data Sheet
RBRC11179
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11179 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Gt(ROSA)26Sor<tm1.1(CAG-tdTomato)Imayo> |
| Former Common name | R26-CAG-LoxP-tdTom |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Itaru Imayoshi, Kyoto University in 2021 and generated in collaboration with Hiroyuki Okuno, Kagoshima University. EGR-101 ES cells derived from C57BL/6N were used to generate this strain. |
| Strain description | tdTomato fluorescent protein is expressed after both Cre-and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, tdTomato is expressed under CAG promoter and WPRE sequence is inserted into 3’ UTR region. FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain. |
| Colony maintenance | |
| References | |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/01/07Room:3-BRack:HSentinel mouse program 2024/10/07Room:3-BRack:HSentinel mouse program 2024/04/08Room:3-BRack:HSentinel mouse program 2024/01/09Room:3-BRack:HSentinel mouse program 2023/10/10Room:3-BRack:HSentinel mouse program 2023/07/10Room:3-BRack:HSentinel mouse program 2023/04/10Room:3-BRack:HSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| GH | Growth hormone polyA (Bovine) | 6 | GH | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1.1(CAG-tdTomato)Imayo> | targeted mutation 1.1, Itaru Imayoshi | |||
| T2A | Thosea asigna virus 2A peptide (insect) | 6 | T2A | ||||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | 6 | WPRE | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 6 | mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter | ||||
| tdTomato | tandem dimeric Red Fluorescent Protein (Discosoma sp.) | 6 | tdTomato | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), Bacteriophage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E.coli neo, Bovine Growth hormone polyA, Yeast FRT (flippase recombination target) site, Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE), Red Fluorescent Protein (Discosoma sp.) tdTomato (tandem dimeric Tomato), T2A (Thosea asigna virus 2A peptide (insect)) |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail:imayoshi.itaru.2n@kyoto-u.ac.jp). (No approval form (Form D) is necessary). |
| Depositor | Itaru Imayoshi (Kyoto University) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
| No Data |