Strain Data Sheet
RBRC11185
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11185 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Gt(ROSA)26Sor<tm1.1(CAG-tdiRFP713NLS)Imayo> |
| Former Common name | R26-CAG-LoxP-iRFP713NLS |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Itaru Imayoshi, Kyoto University in 2021 and generated in collaboration with Hiroyuki Okuno, Kagoshima University. EGR-101 ES cells derived from C57BL/6N were used to generate this strain. |
| Strain description | tdiFP713NLS fluorescent protein is expressed after both Cre-and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, tdiFP713NLS is expressed under CAG promoter and WPRE sequence is inserted into 3’ UTR region. FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain. The HA-epitope tga was is attched to tdiFP713NLS. |
| Colony maintenance | |
| References | |
Health Report | |
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| Examination Date / Room / Rack | 2024/06/10Room:3-2Rack:DSentinel mouse program 2024/03/11Room:3-2Rack:DSentinel mouse program 2023/12/11Room:3-2Rack:DSentinel mouse program 2023/09/11Room:3-2Rack:DSentinel mouse program 2023/06/13Room:3-2Rack:DSentinel mouse program |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| GH | Growth hormone polyA (Bovine) | 6 | GH | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1.1(CAG-tdiRFP713NLS)Imayo> | targeted mutation 1.1, Itaru Imayoshi | |||
| HA | human influenza virus HA (hemagglutinin) tag sequence | 6 | HA | ||||
| T2A | Thosea asigna virus 2A peptide (insect) | 6 | T2A | ||||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | 6 | WPRE | ||||
| iRFP713 | iRFP713 (humanized iRFP713) | 6 | iRFP713 | ||||
| iRFP713 | iRFP713 (humanized iRFP713) | 6 | iRFP713 | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 6 | mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter | ||||
| nls | SV40 nuclear localaization signal (chemically-synthesized nucleotide) | 6 | nls | ||||
| nls | SV40 nuclear localaization signal (chemically-synthesized nucleotide) | 6 | nls | ||||
| tdiRFP713 | tdiRFP713 (tandem dimeric iRFP713) | 6 | tdiRFP713 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| SV40 polyA | 6 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), Bacteriophage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli neomycin resistance gene, pA, bovine GH polyA, SV40 polyA signal, rabbit beta-globin polyA, Yeast FRT (flippase recombination target) site, Near-InfraRed Fluorescent Protein (Rhodopseudomonas palustris) tdiRFP713 (tandem dimeric iRFP713), SV40 nuclear localaization signal (chemically-synthesized nucleotide), Thosea asigna virus 2A peptide (insect), human influenza virus HA (hemagglutinin) tag sequence, Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail:imayoshi.itaru.2n@kyoto-u.ac.jp). (No approval form (Form D) is necessary). |
| Depositor | Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |