Strain Information | |
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Image | |
BRC No. | RBRC11187 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Gt(ROSA)26Sor<tm1.3(CAG-tdiRFP713NLS)Imayo> |
Former Common name | R26-CAG-iRFP713NLS |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Itaru Imayoshi, Kyoto University in 2021 and generated in collaboration with Hiroyuki Okuno, Kagoshima University. EGR-101 ES cells derived from C57BL/6N were used to generate this strain. |
Strain description | tdiFP713NLS fluorescent protein is expressed after both Cre-and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, tdiFP713NLS is expressed under CAG promoter and WPRE sequence is inserted into 3’ UTR region. FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain. Then, loxP-Stop-loxP cassette was removed by injecting pIC-Cre expression plasmid to the fertilized eggs. Therefore, tdiFP713NLS is expressed by all cells throughout the body. The HA-epitope tga was is attched to tdiFP713NLS. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.3(CAG-tdiRFP713NLS)Imayo>targeted mutation 1.3, Itaru Imayoshi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequence6HA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 nuclear localaization signal (chemically-synthesized nucleotide)6nls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tdiRFP713tdiRFP713 (tandem dimeric iRFP713)6tdiRFP713CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tpASV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal6tpA |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sequence, Yeast FRT (flippase recombination target) site, Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE), Near-InfraRed Fluorescent Protein (Rhodopseudomonas palustris) tdiRFP713 (tandem dimeric iRFP713), SV40 nuclear localaization signal (chemically-synthesized nucleotide), human influenza virus HA (hemagglutinin) tag sequence |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail:imayoshi.itaru.2n@kyoto-u.ac.jp). (No approval form (Form D) is necessary). |
Depositor | Itaru Imayoshi (Kyoto University) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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