Strain Data Sheet

RBRC11193

Strain Information

Image
BRC No.RBRC11193
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm2(Asxl1<*>)Toki>
Former Common nameAsxl1-MT KI
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeposited by Toshio Kitamura in 2020. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Mice were further backcrossed to C57BL/6J for 6 generations. C57BL/6J congenic line.
Strain descriptionKnockin mouse strain at the ROSA26 locus in which Asxl1-MT IRES EGFP can be overexpressed in a Cre-dependent manner.
Colony maintenance
References
Expression of mutant Asxl1 perturbs hematopoiesis and promotes susceptibility to leukemic transformation.
Nagase R, Inoue D, Pastore A, Fujino T, Hou H A, Yamasaki N, Goyama S, Saika M, Kanai A, Sera Y, Horikawa S, Ota Y, Asada S, Hayashi Y, Kawabata K C, Takeda R, Tien H F, Honda H, Abdel-Wahab O, Kitamura T
J. Exp. Med., 215(6):1729-1747 (2018). 29643185

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Asxl1
MGI:2684063		ASXL transcriptional regulator 16Asxl1
  • Bohring-Opitz syndrome(DisGeNET, MedGEN)

  • myelodysplastic syndrome(DisGeNET, MedGEN)
    • EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP
    FLAG FLAG tag (synthetic)6FLAG
    Frt yeast FRT (flippase recombination target) site6Frt
    Frt yeast FRT (flippase recombination target) site6Frt
    Gt(ROSA)26Sor
    MGI:104735    		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2(Asxl1<*>)Toki> targeted mutation 2, Toshio Kitamura
    Ires internal ribosomal entry site (EMCV)6
    SA Splice acceptor (adenovirus)6SA
    loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    neo neomycin resistance gene (E. coli)6 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    SV40 virus Stop6

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNASA (Adenovirus Splicing acceptor), P1 phage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, SV40 virus Stop, FLAG tag (synthetic), Mouse Asxl1 (1-1,890) cDNA, yeast FRT (flippase recombination target) sites, IRES (internal ribosomal entry site (EMCV)), jellyfish EGFP cDNA, bovine growth hormone polyA, mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 215(6):1729-1747 (2018). For use of the BIOLOGICAL RESOURCE by a company and a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorToshio Kitamura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data