Strain Data Sheet
RBRC11280
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11280 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6D2-Tg(HTT<*>/EGFP)150Nn |
| Former Common name | HD150QG |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Nobuyuki Nukina, RIKEN in 2021. B6D2F1 mixed genetic background. Natural mating and breeding are not practical. Reproductive technique (IVF) is necessary to maintain this strain. It is important to use mouse that has been confirmed to have the expected repeat length by PCR. |
| Strain description | Transgenic mice harboring human HTT promoter and exon1 with CAG repeats (about 150 repeats) fused with EGFP. This lines was derived from the parental line containing about 190 repeats. Most mice die by 50 weeks. |
| Colony maintenance | |
| References | Decreased expression of hypothalamic neuropeptides in Huntington disease transgenic mice with expanded polyglutamine-EGFP fluorescent aggregates. Kotliarova S, Jana N R, Sakamoto N, Kurosawa M, Miyazaki H, Nekooki M, Doi H, Machida Y, Wong H K, Suzuki T, Uchikawa C, Kotliarov Y, Uchida K, Nagao Y, Nagaoka U, Tamaoka A, Oyanagi K, Oyama F, Nukina N J. Neurochem., 93(3):641-53 (2005). 15836623Depletion of p62 reduces nuclear inclusions and paradoxically ameliorates disease phenotypes in Huntington's model mice. Kurosawa M, Matsumoto G, Kino Y, Okuno M, Kurosawa-Yamada M, Washizu C, Taniguchi H, Nakaso K, Yanagawa T, Warabi E, Shimogori T, Sakurai T, Hattori N, Nukina N Hum. Mol. Genet., 24(4):1092-105 (2015). 25305080 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | ||||
| SV40 polyA | UN | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | human HTT promoter, human Huntingtin (HTT) DNA, jellyfish EGFP cDNA, SV40 polyA |
| Research application | Fluorescent Proteins/lacZ System Mouse Models for Human Disease |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Neurochem. 2005 May;93(3):641-53. doi: 10.1111/j.1471-4159.2005.03035.x. |
| Depositor | Nobuyuki Nukina (RIKEN CBS) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
|---|
| No Data |