Strain Data Sheet

RBRC11283

Strain Information

Image
BRC No.RBRC11283
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Scn4b-Venus)#Nn
Former Common nameScn4b-Venus
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Nobuyuki Nukina, RIKEN in 2021. B6D2F1 were used to generate this transgenic mice. The mice were further backcrossed to C57BL/6J for 9 generations. C57BL/6J congenic line.
Strain descriptionTransgenic mice expressing Venus under the control of the Scn4b gene promoter.
Colony maintenance
References
Singular localization of sodium channel beta4 subunit in unmyelinated fibres and its role in the striatum.
Miyazaki H, Oyama F, Inoue R, Aosaki T, Abe T, Kiyonari H, Kino Y, Kurosawa M, Shimizu J, Ogiwara I, Yamakawa K, Koshimizu Y, Fujiyama F, Kaneko T, Shimizu H, Nagatomo K, Yamada K, Shimogori T, Hattori N, Miura M, Nukina N
Nat. Commun., 5:5525 (2014). 25413837

FACS-array-based cell purification yields a specific transcriptome of striatal medium spiny neurons in a murine Huntington disease model.
Miyazaki H, Yamanaka T, Oyama F, Kino Y, Kurosawa M, Yamada-Kurosawa M, Yamano R, Shimogori T, Hattori N, Nukina N
J. Biol. Chem., 295(29):9768-9785 (2020). 32499373

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Venus Yellow Fluorescent Protein (Aequorea victoria)UNVenus mouse Sodium channel beta4 subunit (Scn4b)promoter
SV40 intron, SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse Sodium channel beta4 subunit (Scn4b)promoter, jellyfish Venus cDNA, SV40 intron, SV40 polyA signal
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2014 Nov 21;5:5525. doi: 10.1038/ncomms6525.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorNobuyuki Nukina (RIKEN CBS)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data