Strain Data Sheet
RBRC11291
Strain Information | |
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| Image | |
| BRC No. | RBRC11291 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129-Lyn<tm1Tya> |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Tadashi Yamamoto, OIST, in 2021. E14 ES Cells were used to generate this strain. Mice were further backcrossed to C57BL/6J for 7 or 8 generations. C57BL/6 congenicc strain. |
| Strain description | Lyn knockout mice. Exon 4 of the Lyn gene was replaced with a neo gene. |
| Colony maintenance | |
| References | Impaired proliferation of peripheral B cells and indication of autoimmune disease in lyn-deficient mice. Nishizumi H, Taniuchi I, Yamanashi Y, Kitamura D, Ilic D, Mori S, Watanabe T, Yamamoto T Immunity, 3, 549-60 (1995). 7584145Impaired tyrosine phosphorylation and Ca2+ mobilization, but not degranulation, in lyn-deficient bone marrow-derived mast cells. Nishizumi H, Yamamoto T J. Immunol., 158, 2350-2355 (1997). 9036984A double-edged kinase Lyn: a positive and negative regulator for antigen receptor-mediated signals. Nishizumi H, Horikawa K, Mlinaric-Rascan I, Yamamoto T J. Exp. Med., 187, 1343-1348 (1998). 9547345 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| GH | Growth hormone polyA (Bovine) | 4 | GH | ||||
| Lyn MGI:96892 | LYN proto-oncogene, Src family tyrosine kinase | 4 | Lyn<tm1Tya> | targeted mutation 1, Tadashi Yamamoto | |||
| neo | neomycin resistance gene (E. coli) | 4 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, bovine growth hormone polyA, mouse Lyn genomic DNA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity 3, 549-60 (1995). |
| Depositor | Tadashi Yamamoto (Okinawa Institute of Science and Technology Graduate University (OIST)) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |