Strain Data Sheet

RBRC11346

Strain Information

Image
BRC No.RBRC11346
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Actb<tm1.1(tetO-GFP/bPAC)Yksk>
Former Common nametetO-GFP-2A-bPAC
H-2 Haplotype
ES Cell lineG0G1
Background strain
Appearance
Strain developmentDeposited by Ryuta Koyama, The University of Tokyo in 2021.
Strain descriptiontetO-GFP-2A-bPAC
Colony maintenance
References
Astrocytic cAMP modulates memory via synaptic plasticity.
Zhou Z, Okamoto K, Onodera J, Hiragi T, Andoh M, Ikawa M, Tanaka K F, Ikegaya Y, Koyama R
Proc. Natl. Acad. Sci. USA, 118(3) e2016584118 (2021). 33452135

Optical manipulation of local cerebral blood flow in the deep brain of freely moving mice.
Abe Y, Kwon S, Oishi M, Unekawa M, Takata N, Seki F, Koyama R, Abe M, Sakimura K, Masamoto K, Tomita Y, Okano H, Mushiake H, Tanaka K F
Cell Rep., 36(4):109427(2021). 34320360

Separate optogenetic manipulation of Nerve/glial antigen 2 (NG2) glia and mural cells using the NG2 promoter.
Mitsuhiro Oishi, Stefan Passlick, Yoshihiko Yamazaki, Miyuki Unekawa, Ruka Adachi, Mayumi Yamada, Itaru Imayoshi, Yoshifumi Abe, Christian Steinhäuser, Kenji F Tanaka
Glia, 71(2) 317-333 (2023). 36165697

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Actb
MGI:87904		actin, beta5Actb<tm1.1(tetO-GFP/bPAC)Yksk> targeted mutation 1.1, Ryuta Koyama
  • autosomal dominant nonsyndromic hearing loss 20(MedGEN)

  • Baraitser-Winter syndrome 1(MedGEN)

  • Baraitser-winter syndrome 2(MedGEN)
    • more 1 Diseases
  • developmental malformations-deafness-dystonia syndrome(MedGEN)
    • EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)5EGFP E. coli tetO (tetracycline operator), CMV promoter
    Frt yeast FRT (flippase recombination target) site5Frt
    T2A Thosea asigna virus 2A peptide (insect)5T2A
    loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP
    loxP phage P1 loxP5loxP
    SV40 polyA5
    photoactivated adenylyl cyclase (bPAC) Beggiatoa sp.5

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAE. coli tetR（tetracycline repressor), E. coli tetO (tetracycline operator), CMV promoter, rabbit Beta globin intron, jellyfish EGFP cDNA, Thosea asigna virus 2A peptide (insect), Beggiatoa sp. photoactivated adenylyl cyclase (bPAC), SV40 polyA signal, Phage P1 loxP sites, Yeast FRT site, mouse Actb genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA. Jan 19;118(3) (2021) :e2016584118. doi: 10.1073/pnas.2016584118.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a research. The RECIPIENT must contact the DEPOSITOR in the case of a application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorRyuta Koyama (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data