Strain Data Sheet
RBRC11346
Strain Information | |
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| Image | |
| BRC No. | RBRC11346 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Actb<tm1.1(tetO-GFP/bPAC)Yksk> |
| Former Common name | tetO-GFP-2A-bPAC |
| H-2 Haplotype | |
| ES Cell line | G0G1 |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Ryuta Koyama, The University of Tokyo in 2021. |
| Strain description | tetO-GFP-2A-bPAC |
| Colony maintenance | |
| References | Glia, 71(2) 317-333 (2023). 36165697 Proc. Natl. Acad. Sci. USA, 118(3) e2016584118 (2021). doi: 10.1073/pnas.2016584118. 33452135 Cell Rep., 36(4):109427(2021). 34320360 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)5EGFPE. coli tetO (tetracycline operator), CMV promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site5Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter T2AThosea asigna virus 2A peptide (insect)5T2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter photoactivated adenylyl cyclase (bPAC) Beggiatoa sp.5 |
Ordering Information | |
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| Donor DNA | E. coli tetR（tetracycline repressor), E. coli tetO (tetracycline operator), CMV promoter, rabbit Beta globin intron, jellyfish EGFP cDNA, Thosea asigna virus 2A peptide (insect), Beggiatoa sp. photoactivated adenylyl cyclase (bPAC), SV40 polyA signal, Phage P1 loxP sites, Yeast FRT site, mouse Actb genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA. Jan 19;118(3) (2021) :e2016584118. doi: 10.1073/pnas.2016584118.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a research. The RECIPIENT must contact the DEPOSITOR in the case of a application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Ryuta Koyama (The University of Tokyo) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |