Strain Data Sheet

RBRC11355

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC11355
Type	CRISPR/Cas9 (Transgene)
Species	Mus musculus
Strain name	B6.Cg-Clec7a<tm1Yiw> Card9<em1Yiw>
Former Common name	Dectin-1/CARD9 dKO/B6
H-2 Haplotype
ES Cell line	E14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain development	Deposited by Yoichiro Iwakura, Tokyo University of Science, in 2022. Mice were generated using fertilized eggs derived from RBRC05094 B6;129P2-Clec7a<tm1Yiw> with the CRISPR/Cas9. C57BL/6J congenic strain.
Strain description	Clec7a/Card9 double mutant mice. 17bp deletion at the Clec7a gene. Clec7a<tm1Yiw> is derived from RBRC05094 B6;129P2-Clec7a<tm1Yiw>.
Colony maintenance
References	Nat. Immunol., 8, 39-46 (2007). 17159982

Health Report
Examination Date / Room / Rack

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Card9caspase recruitment domain family, member 92Card9<em1Yiw>endonuclease-mediated mutation 1, Yoichiro Iwakura Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Clec7aC-type lectin domain family 7, member a6Clec7a<tm1Yiw>targeted mutation 1, Yoichiro Iwakura Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Card9caspase recruitment domain family, member 92Card9<em1Yiw>endonuclease-mediated mutation 1, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Clec7aC-type lectin domain family 7, member a6Clec7a<tm1Yiw>targeted mutation 1, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information
Donor DNA	P1 Phage loxP site, mouse Phosphoglycerate kinase (PGK) promoter, E.coli Neomycin resistant gene, Bovine growth hormone polyadenylation site, mouse Clec7a genomic DNA
Research application	Cre/loxP system
Specific Term and Conditions	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Depositor	Yoichiro Iwakura (Tokyo University of Science)
Strain Status	Frozen embryos
Strain Availability	Recovery and QC required prior to distribution
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data