Strain Data Sheet

RBRC11355

Strain Information

Image
BRC No.RBRC11355
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6.Cg-Clec7a<tm1Yiw> Card9<em1Yiw>
Former Common nameDectin-1/CARD9 dKO/B6
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Yoichiro Iwakura, Tokyo University of Science, in 2022. Mice were generated using fertilized eggs derived from RBRC05094 B6;129P2-Clec7a<tm1Yiw> with the CRISPR/Cas9. C57BL/6J congenic strain.
Strain descriptionClec7a/Card9 double mutant mice. 17bp deletion at the Clec7a gene. Clec7a<tm1Yiw> is derived from RBRC05094 B6;129P2-Clec7a<tm1Yiw>.
Colony maintenance
References
Dectin-1 is required for host defense against Pneumocystis carinii but not against Candida albicans.
Shinobu Saijo, Noriyuki Fujikado, Takahisa Furuta, Soo-hyun Chung, Hayato Kotaki, Keisuke Seki, Katsuko Sudo, Shizuo Akira, Yoshiyuki Adachi, Naohito Ohno, Takeshi Kinjo, Kiwamu Nakamura, Kazuyoshi Kawakami, Yoichiro Iwakura
Nat. Immunol., 8, 39-46 (2007). 17159982

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Card9
MGI:2685628		caspase recruitment domain family, member 92Card9<em1Yiw> endonuclease-mediated mutation 1, Yoichiro Iwakura
  • predisposition to invasive fungal disease due to CARD9 deficiency(MedGEN)
    • Clec7a
    MGI:1861431    		C-type lectin domain family 7, member a6Clec7a<tm1Yiw>
    MGI:3695711    		targeted mutation 1, Yoichiro Iwakura
  • aspergillosis, susceptibility to(MedGEN)

  • candidiasis, familial, 4(MedGEN)
    • loxP phage P1 loxP6loxP
    neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP site, mouse Phosphoglycerate kinase (PGK) promoter, E.coli Neomycin resistant gene, Bovine growth hormone polyadenylation site, mouse Clec7a genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorYoichiro Iwakura (Tokyo University of Science)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data