Strain Information | |
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Image | |
BRC No. | RBRC11364 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6-Gad1<tm1(tetO)Ksak> |
Former Common name | Gad1-Stop-tetO knock-in mice |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Yuchio Yanagawa, Gunma University, and Kenji Sakimura, Niigata University in 2017. This strain was generated using RENKA ES cells derived from C57BL/6NCrlCrlj. Homozygous mice are neonatally lethal. |
Strain description | Floxed stop cassette followed by tetO was knockined between the promoter and coding sequence of the Gad1. |
Colony maintenance | |
References | Mol. Brain, 14(1):5 (2021). 33413507 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HIS3His3 (S. cerevisiae)2HIS3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2E. coli EM7 promoter, Mouse Phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tetOtetracycline operator (E. coli)2tetO Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA2 |
Ordering Information | |
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Donor DNA | P1 ファージ loxP, SV40ウイルス polyA, 酵母 frt, synthetic bacterial EM7 promoter (T7 phage), 大腸菌Neo(neomycin耐性遺伝子), 酵母 HIS3ターミネーター, 大腸菌 tetO配列, マウス Pgk promoter |
Research application | Cre/loxP system FLP/frt system Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain. 2021 Jan 7;14(1):5.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of not a not-for profit institution for a not-for-profit research. |
Depositor | Yuchio Yanagawa (Gunma University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |