Strain Data Sheet

RBRC11365

Strain Information

Image
BRC No.RBRC11365
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gad1<tm1.1(tTA2)Kftnk>
Former Common nameGad1-tTA knock-in mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Gunma University, and Junichi Nakai, Tohoku University in 2006. Homozygous mice are neonatally lethal.
Strain descriptiontTA2 knockin mice at the Gad1 locus.
Colony maintenance
References
Predicting phenolic acid absorption in Caco-2 cells: a theoretical permeability model and mechanistic study.
Farrell T L, Poquet L, Dew T P, Barber S, Williamson G
Cell Report 2, 397-406, 2012 22096083

Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice.
Miyata S, Kakizaki T, Fujihara K, Obinata H, Hirano T, Nakai J, Tanaka M, Itohara S, Watanabe M, Tanaka K F, Abe M, Sakimura K, Yanagawa Y
Mol. Brain, 14(1):5 (2021). 33413507

Health Report

Examination Date / Room / Rack2023/12/11Room:3-5Rack:CSentinel mouse program
2023/09/11Room:3-5Rack:CSentinel mouse program
2023/06/13Room:3-5Rack:CSentinel mouse program
2023/05/22Room:3-5Rack:CAdditional tests

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gad1
MGI:95632		glutamate decarboxylase 12Gad1<tm1.1(tTA2)Kftnk>
MGI:5439170		targeted mutation 1.1, Kenji Tanaka
  • developmental and epileptic encephalopathy 89(MedGEN)
    • loxP phage P1 loxP2loxP
    tTA2 synthetic modified tetracycline-regulated transactivator: E. coli TetR (the repressor of tetracycline resistance operon), herpes simplex virus VP16 transcription avtivating domain (C-terminal portion)2tTA2
    SV40 polyA2

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAtTA2（synthetic modified tetracycline-regulated transactivator: E. coli TetR (the repressor of tetracycline resistance operon), herpes simplex virus VP16 transcription avtivating domain (C-terminal portion)(Urlinger et al., 2000)), SV40 polyA, phage P1 loxP site, mouse Gad1 genomic DNA
    Research applicationCre/loxP system
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain, 14(1):5 (2021). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of not a not-for profit institution for a not-for-profit research.
    DepositorYuchio Yanagawa (Gunma University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data