Strain Data Sheet

RBRC11375

Strain Information

Image
BRC No.RBRC11375
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6.B6D2-Hmces<em1Nsas>
Former Common nameB6-Hmces<em1>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Nobuya Sasaki, Kitasato University in 2021. This strain was generated using fertilized eggs derived from BDF1. Mice were further backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic strain.
Strain descriptionHmces mutants generated by the CRISPR/Cas9. 1 bp insertion at exon 2 of the Hmces gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hmces
MGI:1914053		5-hydroxymethylcytosine (hmC) binding, ES cell specific6Hmces<em1Nsas> endonuclease-mediated mutation 1, Nobuya Sasaki

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAS. pyogenes Single guide RNA, S. pyogenes Humanized Cas9* gene mRNA, Mouse Hmces gene RNA flagment (Single quide RNA)* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research applicationMetabolism Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration.
DepositorNobuya Sasaki (Kitasato University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data