Strain Information | |
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Image | |
BRC No. | RBRC11377 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | C57BL/6-Atf5<em1(Atf5/HA)Yuta> |
Former Common name | ATF5-HA knock-in, B6-ATF5HA KI |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Haruo Nakano, Tokyo university of the pharmacy and life sciences in 2019.The HA-tag was introduced into zygotes by CRISPR/Cas9 genome editing. C57BL/6NJcl (CLEA Japan Inc.)The ATF5-HA knock-in male G0 offspring was mated with wild type females, and then propageted by sibling crossing (Heterozygous x Heterozygous or Homozygous x Homozygous). |
Strain description | ATF5-Hemagglutinin (HA) knock-in mice. A HA-tag sequence was integrated into the stop codon site of ATF5 gene at an in-flame position. |
Colony maintenance | |
References | Cell Tissue Res., (2021). doi: 10.1007/s00441-021-03450-7. 33825962 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Atf5activating transcription factor 57Atf5<em1(Atf5/HA)Yuta>endonuclease-mediated mutation 1, Yuji Takahashi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequence7HA |
Ordering Information | |
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Donor DNA | influenza virus HA (hemagglutinin) tag sequence |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Tissue Res. 2021 Apr 7. doi: 10.1007/s00441-021-03450-7.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Yuji Takahashi (Tokyo University of Pharmacy and Life Sciences) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |