Strain Data Sheet
RBRC11378
Strain Information | |
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| Image | |
| BRC No. | RBRC11378 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | ICR-Atf5<em2(Atf5/HA)Yuta> |
| Former Common name | ATF5-HA knock-in, ICR-ATF5HA KI |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Haruo Nakano, Tokyo university of the pharmacy and life sciences in 2019.The HA-tag was introduced into zygotes by CRISPR/Cas9 genome editing. Jcl:ICR (CLEA Japan Inc.)The ATF5-HA knock-in male G0 offspring was mated with wild type females, and then propageted by sibling crossing (Heterozygous x Heterozygous or Homozygous x Homozygous). |
| Strain description | ATF5-Hemagglutinin (HA) knock-in mice. A HA-tag sequence was integrated into the stop codon site of ATF5 gene at an in-flame position. |
| Colony maintenance | |
| References | Functional validation of epitope-tagged ATF5 knock-in mice generated by improved genome editing of oviductal nucleic acid delivery (i-GONAD). Nakano H, Kawai S, Ooki Y, Chiba T, Ishii C, Nozawa T, Utsuki H, Umemura M, Takahashi S, Takahashi Y Cell Tissue Res., (2021). 33825962 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Atf5 MGI:2141857 | activating transcription factor 5 | 7 | Atf5<em2(Atf5/HA)Yuta> | endonuclease-mediated mutation 2, Yuji Takahashi | |||
| HA | human influenza virus HA (hemagglutinin) tag sequence | 7 | HA | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | influenza virus HA (hemagglutinin) tag sequence |
| Research application | |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Tissue Res. 2021 Apr 7. doi: 10.1007/s00441-021-03450-7.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Yuji Takahashi (Tokyo University of Pharmacy and Life Sciences) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |