Strain Data Sheet

RBRC11386

Strain Information

Image
BRC No.RBRC11386
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Tg(Ctsk-OCZF)#Akt
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Akiko Kukita, Saga University in 2021. This strain was generated using fertilized eggs derived from B6D2F1 and the mice were further backcrossed to C57BL/6N for three generations. Mixed genetic background.
Strain descriptionTransgenic mice expressing rat OCZF/LRF/FBI-1 under the control of mouse cathepsin K promoter.
Colony maintenance
References
Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis.
Kukita A, Kukita T, Ouchida M, Maeda H, Yatsuki H, Kohashi O
Blood, 94(6):1987-97 (1999). 10477728

The transcription factor FBI-1/OCZF/LRF is expressed in osteoclasts and regulates RANKL-induced osteoclast formation in vitro and in vivo.
Kukita A, Kukita T, Nagata K, Teramachi J, Li Y J, Yoshida H, Miyamoto H, Gay S, Pessler F, Shobuike T
Arthritis Rheum., 63(9):2744-54 (2011). 21590684

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
HBB human beta-globinUNHBB
Ires internal ribosomal entry site (EMCV)UN
ZBTB7A zinc finger and BTB domain containing 7a (rat)UNZBTB7A mouse Cathepsin K promoter
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse Cathepsin K promoter, human 5'-donor site from the first intron of the human beta-globin gene and the branch and 3'-acceptor site from the intron of an immunoglobulin gene heavy chain variable region, rat OCZF cDNA, encephalomyocarditis virus, EMCV internal ribosomal entry site (IRES), Aequorea victoria enhanced green fluorescent protein (EGFP)cDNA, SV 40 poly A signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arthritis Rheum., 63(9):2744-54 (2011).
DepositorAkiko Kukita (Saga University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data