Strain Data Sheet

RBRC11387

Strain Information

Image
BRC No.RBRC11387
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pmepa1<em1Akt>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Akikot Kukita, Saga University in 2021. Mice were generated using C57BL/6N. C57BL/6N genetic background.
Strain descriptionPmepa1 mutant mice generated by the CRISPR/Cas9. 1 bp insertion at exon 2 of the Pmepa1 gene.
Colony maintenance
References
Prostate transmembrane protein androgen induced 1 is induced by activation of osteoclasts and regulates bone resorption.
Xu X, Hirata H, Shiraki M, Kamohara A, Nishioka K, Miyamoto H, Kukita T, Kukita A
FASEB J., 33(3):4365-4375 (2019). 30557043

PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking.
Hirata H, Xu X, Nishioka K, Matsuhisa F, Kitajima S, Kukita T, Murayama M, Urano Y, Miyamoto H, Mawatari M, Kukita A
FASEB J., 35(2):e21281 (2021). 33484199

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pmepa1
MGI:1929600		prostate transmembrane protein, androgen induced 12Pmepa1<em1Akt>
MGI:7491928		endonuclease-mediated mutation 1,

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J., 35(2):e21281 (2021).The RECIPIENT must contact the DEPOSITOR in the case of applications for any patents or commercial use base on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkiko Kukita (Saga University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data