Strain Data Sheet

RBRC11393

Strain Information

Image
BRC No.RBRC11393
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ebf3<tm2.1Tng>
Former Common nameEbf3-flox
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeposited by Takashi Nagasawa, Osaka University in 2021. This strain was generated using KY1.1 ES cells derived from 129/B6. Mice were further backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic line.
Strain descriptionEbf3-flox mouse. A pair of loxP flanks Exon 3 and Exon 4 of the Ebf3 gene.
Colony maintenance
References
Stem cell niche-specific Ebf3 maintains the bone marrow cavity.
Seike M, Omatsu Y, Watanabe H, Kondoh G, Nagasawa T
Genes Dev., 32, 359-372 (2018). 29563184

Health Report

Examination Date / Room / Rack2024/10/07Room:3-BRack:KSentinel mouse program
2024/04/08Room:3-BRack:KSentinel mouse program
2024/01/09Room:3-BRack:KSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ebf3
MGI:894289		early B cell factor 37Ebf3<tm2.1Tng> targeted mutation 2.1, Takashi Nagasawa
  • hypotonia, ataxia, and delayed development syndrome(MedGEN)
    • Frt yeast FRT (flippase recombination target) site7Frt
    loxP phage P1 loxP7loxP
    loxP phage P1 loxP7loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, yeast FRT (flippase recombination target) site, mouse Ebf3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 32, 359-372 (2018).RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takashi Nagasawa) and enter into a Material Transfer Agreement with Osaka University. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorTakashi Nagasawa (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data