Strain Data Sheet
RBRC11397
Strain Information | |
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| Image | |
| BRC No. | RBRC11397 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C.129P2(B6)-Il5ra<tm1Ktk> |
| Former Common name | BALB/c IL-5R alpha -/- |
| H-2 Haplotype | |
| ES Cell line | E14.1 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Kiyoshi Takatsu, Institute of Medical Science in 1996. E14.1 ES cells were used. The mutant mice were backcrossed to BALB/c. |
| Strain description | IL-5Ralpha gene knockout mice. A translational stop codon was introduced into exon 5 of the Il5ra and the exon 6 was replaced with a neo cassette. |
| Colony maintenance | |
| References | Defective B-1 cell development and impaired immunity against Angiostrongylus cantonensis in IL-5R alpha-deficient mice. Yoshida T, Ikuta K, Sugaya H, Maki K, Takagi M, Kanazawa H, Sunaga S, Kinashi T, Yoshimura K, Miyazaki J, Takaki S, Takatsu K Immunity, 4(5):483-94 (1996). 8630733Functional dissection of the cytoplasmic subregions of the interleukin-5 receptor alpha chain in growth and immunoglobulin G1 switch recombination of B cells. Moon B G, Yoshida T, Shiiba M, Nakao K, Katsuki M, Takaki S, Takatsu K Immunology, 102(3):289-300 (2001). 11298827 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Il5ra MGI:96558 | interleukin 5 receptor, alpha | 6 | Il5ra<tm1Ktk> MGI:2180501 | targeted mutation 1, Kiyoshi Takatsu | |||
| neo | neomycin resistance gene (E. coli) | 6 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Mouse IL-5R alpha genomic DNA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity 4, 483-494 (1996)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in the articles when the users publish their data using these mice for 1 year after provided. Users should contact the depositor in the case of application for any patents with the results from these mice. |
| Depositor | Kiyoshi Takatsu (Toyama Prefectural Institute for Pharmaceutical Research) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |