Strain Data Sheet

RBRC11399

Strain Information

Image
BRC No.RBRC11399
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Arntl<tm1Shbi>
Former Common nameBmal1<flox>, B6.Cg-Arntl<tm1Shbi>
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Shigeki Shinba, Nihon University in 2021. This strain was generated using Bruce 4 (ES cell) derived from B6.Cg-Thy1.1.
Strain descriptionFloxed Bmal1 (Arntl) mouse. A frt-flanked neo gene exists between exon 8 and 9.
Colony maintenance
References
Deficient of a clock gene, brain and muscle Arnt-like protein-1 (BMAL1), induces dyslipidemia and ectopic fat formation.
Shimba S, Ogawa T, Hitosugi S, Ichihashi Y, Nakadaira Y, Kobayashi M, Tezuka M, Kosuge Y, Ishige K, Ito Y, Komiyama K, Okamatsu-Ogura Y, Kimura K, Saito M
PLoS One. 6(9):e25231 (2011). 21966465

Deletion of Bmal1 Prevents Diet-Induced Ectopic Fat Accumulation by Controlling Oxidative Capacity in the Skeletal Muscle.
Wada T, Ichihashi Y, Suzuki E, Kosuge Y, Ishige K, Uchiyama T, Makishima M, Nakao R, Oishi K, Shimba S
Int. J. Mol. Sci., 19(9):2813 (2018). 30231537

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bmal1
MGI:1096381		basic helix-loop-helix ARNT like 17Bmal1<tm1Shbi> endonuclease mediated mutation 1, Shigeki Simba
Frt yeast FRT (flippase recombination target) site7Frt
GH Growth hormone polyA (Bovine)7GH
loxP phage P1 loxP7loxP
loxP phage P1 loxP7loxP
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, bovine GH polyA, yeast mutated FRT (flippase recombination target) site, mouse Arntl genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorShigeki Simba (Nihon University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data