Strain Information | |
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Image | |
BRC No. | RBRC11400 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | C.Cg-Spib<tm2.1Ksho> |
Former Common name | Spi-B flox BALB |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2005. Backrossed to BALB/cAJcl by Koji Hase, Keio University Faculty of Pharmacy around 2019-2020. |
Strain description | Spi-B is an Ets family transcription factor. In B6.Cg-Spib xxxx mice, two loxP sites are inserted in the introns of the Spi-B gene locus. Expression of cre recombinase leads to deletion of Spi-B coding exon(s) flanked by the loxP sites and disruption of the Spi-B gene locus. Spi-B-deficient mice show various phenotypes, such as defects in plasmacytoid dendritic cell function and development and defective development of an intestinal epithelial cell subset, M cell. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SpibSpi-B transcription factor (Spi-1/PU.1 related)7Spib<tm2.1Ksho>targeted mutation 2.1, Tsuneyasu Kaisho Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Spib genomic DNA |
Research application | Cre/loxP system FLP/frt system Immunology and Inflammation Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval on terms and conditions of use of it with the DEPOSITOR(Dr. Tsuneyasu Kaisho) and CO-DEVELOPER (Dr. Koii Hase). |
Depositor | Tsuneyasu Kaisho (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |