Strain Data Sheet

RBRC11402

Strain Information

Image
BRC No.RBRC11402
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Cdkn1a<em1Matsu>
Former Common namep21 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Makoto Matsuyama, Shigei Medical Research Institute in 2021. C57BL/6H genetic background.
Strain descriptionMutant mice for Cdkn1a. This strain was generated by the CRISPR/Cas9. Stop codon was inserted at exon 2 of the Cdkn1a.
Colony maintenance
References
The p21 dependent G2 arrest of the cell cycle in epithelial tubular cells links to the early stage of renal fibrosis.
Koyano T, Namba M, Kobayashi T, Nakakuni K, Nakano D, Fukushima M, Nishiyama A, Matsuyama M
Sci. Rep., 19;9(1):12059 (2019). 31427681

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cdkn1a
MGI:104556		cyclin dependent kinase inhibitor 1A17Cdkn1a<em1Matsu> endonuclease-mediated mutation 1, Makoto Matsuyama

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNA
Research applicationCell Biology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2019 Aug 19;9(1):12059.
DepositorMakoto Matsuyama (Shigei Medical Research Institute)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data