Strain Data Sheet

RBRC11431

Strain Information

Image
BRC No.RBRC11431
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Flt1<tm1Msh>
Former Common nameFLT1<TK-/-> mice, FLT1TK<+/-> mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeposited by Masabumi Shibuya, Jobu University in 2021. This strain was generated using J1 (ES Cell) derived from 129S4/SvJae. Mice were backcrossed to C57BL.6 for several generations. Mixed genetic background.
Strain descriptionFlt1 mutant mice lacking kinase domain.
Colony maintenance
References
Flt-1 lacking the tyrosine kinase domain is sufficient for normal development and angiogenesis in mice.
Hiratsuka S, Minowa O, Kuno J, Noda T, Shibuya M
Proc. Natl. Acad. Sci. USA, 95(16):9349-54 (1998). 9689083

Vascular endothelial growth factor receptor-1 signaling promotes mobilization of macrophage lineage cells from bone marrow and stimulates solid tumor growth.
Muramatsu M, Yamamoto S, Osawa T, Shibuya M
Cancer Res., 70(20):8211-21 (2010). 20924106

Signaling of vascular endothelial growth factor receptor-1 tyrosine kinase promotes rheumatoid arthritis through activation of monocytes/macrophages.
Murakami M, Iwai S, Hiratsuka S, Yamauchi M, Nakamura K, Iwakura Y, Shibuya M
Blood, 108(6):1849-56 (2006). 16709927

A Functional Role for VEGFR1 Expressed in Peripheral Sensory Neurons in Cancer Pain.
Selvaraj D, Gangadharan V, Michalski C W, Kurejova M, Stosser S, Srivastava K, Schweizerhof M, Waltenberger J, Ferrara N, Heppenstall P, Shibuya M, Augustin H G, Kuner R
Cancer Cell., 27(6):780-96 (2015). 26058077

MMP9 induction by vascular endothelial growth factor receptor-1 is involved in lung-specific metastasis.
Hiratsuka S, Nakamura K, Iwai S, Murakami M, Itoh T, Kijima H, Shipley J M, Senior R M, Shibuya M
Cancer Cell, 2(4):289-300 (2002). 12398893

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Flt1FMS-like tyrosine kinase 15Flt1targeted mutation 1, Masabumi Shibuya
neoneomycin resistance gene (E. coli)5mouse Phosphoglycerate kinase (PGK) promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse Phosphoglycerate kinase (PGK) promoter, E. coli Neomycin-resistant gene, Mouse FLT1 gene: Part of Intron-16, -17 genomic DNA
Research applicationMouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA., 95(16):9349-54 (1998). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. (from the Deposition time on 2021 year to 2023y.3.31. After that, there is no requirement for this item.)
DepositorMasabumi Shibuya (Jobu University)
Strain Statusan icon for Live miceLive mice
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data