Strain Data Sheet
RBRC11436
Strain Information | |
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| Image | |
| BRC No. | RBRC11436 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129S6-Islr<tm1.1(cre/ERT2)Mat> |
| Former Common name | Meflin-CreERT2 |
| H-2 Haplotype | |
| ES Cell line | CSL3[129S6/SvEvTac] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Atsushi Enomoto and Masahide Takahashi, Nagoya University in 2021. This strain was generated using CSL3 (ES cell) derived from 129S6/SvEvTac and mice were backcrossed to C57BL/6J for more than 8 generations. |
| Strain description | Cre/ERT2 konckin mice in which an open reading frame of the Islr gene was replaced with Cre/ERT2. |
| Colony maintenance | |
| References | Meflin-Positive Cancer-Associated Fibroblasts Inhibit Pancreatic Carcinogenesis. Mizutani Y, Kobayashi H, Iida T, Asai N, Masamune A, Hara A, Esaki N, Ushida K, Mii S, Shiraki Y, Ando K, Weng L, Ishihara S, Ponik S M, Conklin M W, Haga H, Nagasaka A, Miyata T, Matsuyama M, Kobayashi T, Fujii T, Yamada S, Yamaguchi J, Wang T, Woods S L, Worthley D, Shimamura T, Fujishiro M, Hirooka Y, Enomoto A, Takahashi M Cancer Res., 79(20):5367-5381 (2019). 31439548 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| ERT2 | estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A) | 9 | ERT2 | ||||
| Frt | yeast FRT (flippase recombination target) site | 9 | Frt | ||||
| HBB | human beta-globin polyA | 9 | HBB | ||||
| Islr MGI:1349645 | immunoglobulin superfamily containing leucine-rich repeat | 9 | Islr<tm1.1(cre/ERT2)Mat> | targeted mutation 1.1,Masahide Takahashi | |||
| cre | Phage P1 Cre recombinase | 9 | cre | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | P1 bacteriophage Cre, Human ERT2 cDNA, Human beta-globin polyA, Saccharomyces cerevisiae FRT site, Mouse Islr (knock-in)genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Res. 2019 Oct 15;79(20):5367-5381. doi: 10.1158/0008-5472.CAN-19-0454.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT shall reach an agreement with the DEPOSITOR on terms and conditions of use of the BIOLOGICAL RESOURCE and shall obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for an patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Atsushi Enomoto (Nagoya University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jun 2022 |
BRC mice in Publications |
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| No Data |