Strain Information | |
---|---|
Image | |
BRC No. | RBRC11437 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S6-Islr<tm1.1(ZsGreen/DTR/cre)Mat> |
Former Common name | Meflin-ZDC |
H-2 Haplotype | |
ES Cell line | CSL3[129S6/SvEvTac] |
Background strain | |
Appearance | |
Strain development | Deposited by Atsushi Enomoto and Masahide Takahashi, Nagoya University in 2021. This strain was generated using CSL3 (ES cell) derived from 129S6/SvEvTac and then backcrossed to C57BL/6J for 6 generations. |
Strain description | Knockin mice harboring ZsGreen-t2a-Diphtheria toxin receptor (DTR)-t2a-Cre by replacing Islr open reading frame. |
Colony maintenance | |
References | Meflin-Positive Cancer-Associated Fibroblasts Inhibit Pancreatic Carcinogenesis. Mizutani Y, Kobayashi H, Iida T, Asai N, Masamune A, Hara A, Esaki N, Ushida K, Mii S, Shiraki Y, Ando K, Weng L, Ishihara S, Ponik S M, Conklin M W, Haga H, Nagasaka A, Miyata T, Matsuyama M, Kobayashi T, Fujii T, Yamada S, Yamaguchi J, Wang T, Woods S L, Worthley D, Shimamura T, Fujishiro M, Hirooka Y, Enomoto A, Takahashi M Cancer Res., 79(20):5367-5381 (2019).doi: 10.1158/0008-5472.CAN-19-0454. 31439548Roles of the Mesenchymal Stromal/Stem Cell Marker Meflin in Cardiac Tissue Repair and the Development of Diastolic Dysfunction. Hara A, Kobayashi H, Asai N, Saito S, Higuchi T, Kato K, Okumura T, Bando Y K, Takefuji M, Mizutani Y, Miyai Y, Saito S, Maruyama S, Maeda K, Ouchi N, Nagasaka A, Miyata T, Mii S, Kioka N, Worthley D L, Murohara T, Takahashi M, Enomoto A Circ Res. Aug 2;125(4):414-430.(2019) doi: 10.1161/CIRCRESAHA.119.314806. 31221024 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
SV40 polyA signal | 9 | ||||||
Frt | yeast FRT (flippase recombination target) site | 9 | Frt | ||||
HBEGF | heparin-binding EGF-like growth factor (human) | 9 | HBEGF | ||||
Islr | immunoglobulin superfamily containing leucine-rich repeat | 9 | Islr | targeted mutation 1.1,Masahide Takahashi | |||
T2A | Thosea asigna virus 2A peptide (insect) | 9 | T2A | ||||
ZsGreen | Green Fluorescent Protein (Zoanthus sp.) | 9 | ZsGreen | ||||
cre | Phage P1 Cre recombinase | 9 | cre |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
---|---|
Donor DNA | Zoanthus sp ZsGreen cDNA, Human DTR cDNA, Thosea asigna virus T2A, P1 bacteriophage Cre, Simian virus 40 PolyA signal, Saccharomyces cerevisiae FRT site, mouse Islr genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Circ Res. 2019 Aug 2;125(4):414-430. doi: 10.1161/CIRCRESAHA.119.314806. Cancer Res. 2019 Oct 15;79(20):5367-5381. doi: 10.1158/0008-5472.CAN-19-0454.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT shall reach an agreement with the DEPOSITOR on terms and conditions of use of the BIOLOGICAL RESOURCE and shall obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for an patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Atsushi Enomoto (Nagoya University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jun 2022 |
BRC mice in Publications |
---|
No Data |