Strain Data Sheet
RBRC11439
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11439 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6;B6D2-Tg(CAG-OsTIR1-P2A-mAID-EGFP-nanoluc)#19Ysa |
| Former Common name | pMK411-#19 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Yumiko Saga, National Institute of Genetics in 2021. This strain was generated using fertilized eggs derived from BDF1, then backcrossed to C57BL/6J twice. |
| Strain description | Transgenic mice (Line 19) expressing EGFP reporter and nanoluc fused with OsTIR and AID. |
| Colony maintenance | |
| References | The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi K I, Kanemaki M T Nat. Commun., 11(1):5701 (2020). 33177522 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/04/08Room:3-ARack:JSentinel mouse program 2024/01/09Room:3-ARack:JSentinel mouse program 2023/10/10Room:3-ARack:JSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | ||||
| NanoLuc | luciferase (Oplophorus gracilirostris) | UN | NanoLuc | ||||
| P2A | porcine teschovirus-1 2A peptide | UN | P2A | ||||
| TIR1 | F-box transport inhibitor response 1 (Oryza sativa) | UN | TIR1 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| mAID | mAID (Arabidopsis thaliana) | UN | mAID | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Oryza sativa TIRI(F74G) cDNA, Porcie teschovirus P2A, Arabidopsis thaliana mAID cDNA, Aequorea victoria EGFP cDNA, Oplophorus gracilirostris luciferase cDNA, SV40 pA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 11(1):5701 (2020). The RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
| Depositor | Yumiko Saga (National Institute of Genetics) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Feb 2022 |
BRC mice in Publications |
|---|
| No Data |