Strain Data Sheet

RBRC11440

Strain Information

Image
BRC No.RBRC11440
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameJF1.129P2(B6)-Kdm6b<tm1.1Masn>
Former Common name
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Masahide Asano, Kyoto University in 2021. Mice were generated using the E14 ES cells derived from 129P2/OlaHsd and the backcrossed to Jf1 for 7 generations.
Strain descriptionJmjd3(Kdm6b)knockout mice in JF1 congenic background. Exon 18 was deleted.
Colony maintenance
References
The ancestor of extant Japanese fancy mice contributed to the mosaic genomes of classical inbred strains.
Takada T, Ebata T, Noguchi H, Keane T M, Adams D J, Narita T, Shin-I T, Fujisawa H, Toyoda A, Abe K, Obata Y, Sakaki Y, Moriwaki K, Fujiyama A, Kohara Y, Shiroishi T
Genome Res., 23(8), 1329-1338 (2013). 23604024

New insights into the role of Jmjd3 and Utx in axial skeletal formation in mice.
Naruse C, Shibata S, Tamura M, Kawaguchi T, Abe K, Sugihara K, Kato T, Nishiuchi T, Wakana S, Ikawa M, Asano M
FASEB J., 31(6):2252-2266 (2017). 28188179

Health Report

Examination Date / Room / Rack2023/07/24Room:3-CRack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site11Frt
Frt yeast FRT (flippase recombination target) site11Frt
Ires internal ribosomal entry site (EMCV)11
Kdm6b
MGI:2448492		KDM1 lysine (K)-specific demethylase 6B11Kdm6b<tm1.1Masn>
MGI:6277129		targeted mutation 1.1, Masahide Asano
  • neurodevelopmental disorder with coarse facies and mild distal skeletal abnormalities(MedGEN)
    • loxP phage P1 loxP11loxP
    neo neomycin resistance gene (E. coli)11

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT sites, Mouse En-2 splicing acceptor, encephalomyocarditis virus Internal ribosomal entry site, E. coli Neomycin resistant gene, Bovine polyA signal, mouse Kdm6b genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J. 2017 Jun;31(6):2252-2266. doi: 10.1096/fj.201600642R. Genome Res. 2013 Aug;23(8):1329-38.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact Kyoto University and National Institute of Genetics in the case of application for an patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Masahide Asano, Dr. Chie Naruse and Dr. Tsuyoshi Koide is requested. (Contact info: Dr. Masahide Asano <asano@anim.med.kyoto-u.ac.jp>, Institute of Laboratory Animals Graduate School of Medicine Kyoto University and Dr. Tsuyoshi Koide Ph.D. <tkoide@nig.ac.jp> Mouse Genomics Resource Laboratory, National Institute of Genetics)
    DepositorMasahide Asano (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data