Strain Data Sheet

RBRC11510

Strain Information

Image
BRC No.RBRC11510
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-4930451I11Rik<em1Nino>
Former Common nameFIMP-KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Naokazu Inoue, Fukushima Medical University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous male mutants are infertile.
Strain description4930451I11Rik mutant mouse generated by the CRISPR/Cas9. Stop codon was inserted immediately after translation initiation codon.
Colony maintenance
References
Deletion of the initial methionine codon of the Tmem95 gene causes subfertility, but not complete infertility, in male mice.
Naokazu Inoue, Ikuo Wada
Biol. Reprod., 106(3):378-381 (2022). 34982145

Deletion of the initial methionine codon of the Tmem95 gene causes subfertility, but not complete infertility, in male mice.
Inoue N, Wada I
Biol. Reprod., 106(3):378-381 (2022). 34982145

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
4930451I11Rik
MGI:1925368		RIKEN cDNA 4930451I11 gene74930451I11Rik<em1Nino> endonuclease-mediated mutation 1, Naokazu Inoue

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorNaokazu Inoue (Fukushima Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data