Strain Data Sheet
RBRC11512
Strain Information | |
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| Image | |
| BRC No. | RBRC11512 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129P2-Utf1<tm1Okud> |
| Former Common name | UTF1 knockout mouse |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Strain development: The targeting vector harboring neomycin resistance gene with SV40 poly-A addition signal under control of the endogenous UTF1 enhance-promoter was transferred to E14 ES cells to replace the 1st exon of UTF1 gene. The resultant UTF1 locus can produce no UTF1 protein (even the truncated form). Developer: Dr. Yo-ichi Nabeshima (National Institute of Neuroscience, Kodaira, Tokyo (1997), Present Affiliation :Foundation for Biomedical Research and Innovation at Kobe, Hyogo)Year of Development: 1997Year of Distribution: 1998 |
| Strain description | The prominent phenotype of UTF1 KO homozygous mice is “in utero growth retardation”. This phenotype is due to the growth retardation of placenta, not due to the defect of the differentiation program of embryo per se. This placental growth defect is unexpected because UTF1 gene is identified as a gene whose expression is pluripotent cell specific (Okuda, A. et al. (1998) EMBO J. 17: 2019–2032). This UTF1 KO phenotype indicates that this mouse strain may be useful to analyze not only the differentiation program of the embryo but also the placental developmental program.Homozygous mutant of C57BL/6J background is embryonic or peri-natal lethal. Some of the homozygous mutant mice of F1 (B6 and ICR) genetic background are viable. This phenotypic difference suggests that the severity of phenotype may be dependent on the genetic background (see Nishimoto,M. et al. (2013) PLoS ONE 8(7): e68119). Appearance: Black [a/a B/B C/C]Colony maintenance: Heterozygote crossing to C57BL/6JJcl |
| Colony maintenance | Backcross C57BL/6J (Jcl) : backcross to male C57BL/6J at least three generations. Homozygous mouse shows the “in utero growth retardation” phenotype. |
| References | UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. A Okuda, A Fukushima, M Nishimoto, A Orimo, T Yamagishi, Y Nabeshima, M Kuro-o, Y i Nabeshima, K Boon, M Keaveney, H G Stunnenberg, M Muramatsu EMBO J. 17: 2019-2032 (1998). 9524124UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Okuda A, Fukushima A, Nishimoto M, Orimo A, Yamagishi T, Nabeshima Y, Kuro-o M, Nabeshima Y i, Boon K, Keaveney M, Stunnenberg H G, Muramatsu M EMBO J. 17: 2019-2032 (1998). 9524124In vivo function and evolution of the eutherian-specific pluripotency marker UTF1. Nishimoto M, Katano M, Yamagishi T, Hishida T, Kamon M, Suzuki A, Hirasaki M, Nabeshima Y, Nabeshima Y, Katsura Y, Satta Y, Deakin J E, Graves J A, Kuroki Y, Ono R, Ishino F, Ema M, Takahashi S, Kato H, Okuda A PLoS One, 8(7):e68119 (2013). 23874519 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Utf1 MGI:1276125 | undifferentiated embryonic cell transcription factor 1 | 7 | Utf1<tm1Okud> MGI:5543270 | targeted mutation 1, Akihiko Okuda | |||
| neo | neomycin resistance gene (E. coli) | 7 | |||||
| SV40 polyA additional signal | 7 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | E. coli Neomycin resistance gene, SV40 PolyA addition signal, mouse Utf1 genomic DNA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 8(7):e68119 (2013). doi: 10.1371/journal.pone.0068119In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Akihiko Okuda (Saitama Medical University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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