Strain Data Sheet
RBRC11513
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11513 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6NJcl.Cg-Clstn1<tm1.1Tymo> |
| Former Common name | Alcadeinα KO; Calsyntenin-1 KO |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Toru Yamamoto, Kagawa University in 2021. This strain was generated using TT2 ES cells derived from (C57BL/6 x CBA)F1. Mice were further backcrossed to C57BL/6N for 10 generations. C57BL/6N congenic strain. Frt-flanked Neo cassette was removed in this strain. |
| Strain description | Clstn1 knockout mice with LacZ reporter replacing Exon 1 of the Clstn1 gene. |
| Colony maintenance | |
| References | EMBO J., 26(6):1475-86 (2007). 17332754 J. Biol. Chem., 295(28):9650-9662 (2020). doi: 10.1074/jbc.RA119.012386. 32467230 Traffic., 13(6):834-48 (2012). 22404616 J. Biol. Chem., 278(49):49448-58 (2003). 12972431 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin 3’ end4HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter frtyeast FRT (flippase recombination target) site4frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox71mutant lox sequence4lox71 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP |
Ordering Information | |
|---|---|
| Donor DNA | phage P1 lox71 site, E.coli LacZ, mouse PGK 3' end, Yeast frt site, rabbit beta-globin 3’end, phage P1 loxP site, mouse Clstn1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 295(28):9650-9662 (2020). doi: 10.1074/jbc.RA119.012386.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Ace.No. CDB0472K and Web. Site (http ://https://large.riken.jp/distribution/mutant-list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (J. Biol. Chem., 295 9650-9662. doi:10.1074/jbc.RA119.012386). Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
| Depositor | Tohru Yamamoto (Kagawa University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
| No Data |