Strain Data Sheet

RBRC11513

update : 2025-02-20

Strain Information
Image
BRC No.	RBRC11513
Type	Targeted Mutation Congenic
Species	Mus musculus
Strain name	B6NJcl.Cg-Clstn1<tm1.1Tymo>
Former Common name	Alcadeinα KO; Calsyntenin-1 KO
H-2 Haplotype
ES Cell line	TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development	Deposited by Toru Yamamoto, Kagawa University in 2021. This strain was generated using TT2 ES cells derived from (C57BL/6 x CBA)F1. Mice were further backcrossed to C57BL/6N for 10 generations. C57BL/6N congenic strain. Frt-flanked Neo cassette was removed in this strain.
Strain description	Clstn1 knockout mice with LacZ reporter replacing Exon 1 of the Clstn1 gene.
Colony maintenance
References	Novel cadherin-related membrane proteins, Alcadeins, enhance the X11-like protein-mediated stabilization of amyloid beta-protein precursor metabolism. Araki Y, Tomita S, Yamaguchi H, Miyagi N, Sumioka A, Kirino Y, Suzuki T J. Biol. Chem., 278(49):49448-58 (2003). 12972431 The novel cargo Alcadein induces vesicle association of kinesin-1 motor components and activates axonal transport. Araki Y, Kawano T, Taru H, Saito Y, Wada S, Miyamoto K, Kobayashi H, Ishikawa H O, Ohsugi Y, Yamamoto T, Matsuno K, Kinjo M, Suzuki T EMBO J., 26(6):1475-86 (2007). 17332754 A small peptide sequence is sufficient for initiating kinesin-1 activation through part of TPR region of KLC1. Kawano T, Araseki M, Araki Y, Kinjo M, Yamamoto T, Suzuki T Traffic., 13(6):834-48 (2012). 22404616 Amyloidogenic processing of amyloid beta protein precursor (APP) is enhanced in the brains of alcadein alpha-deficient mice. Gotoh N, Saito Y, Hata S, Saito H, Ojima D, Murayama C, Shigeta M, Abe T, Konno D, Matsuzaki F, Suzuki T, Yamamoto T J. Biol. Chem., 295(28):9650-9662 (2020). 32467230

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Clstn1 MGI:1929895	calsyntenin 1	4	Clstn1<tm1.1Tymo>	targeted mutation 1.1, Toru Yamamoto
HBB2	rabbit beta-globin 3’ end	4	HBB2
frt	yeast FRT (flippase recombination target) site	4	frt
lacZ	beta-galactosidase (E. coli)	4
lox71	mutant lox sequence	4	lox71
loxP	phage P1 loxP	4	loxP

Phenotype
Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information
Donor DNA	phage P1 lox71 site, E.coli LacZ, mouse PGK 3' end, Yeast frt site, rabbit beta-globin 3’end, phage P1 loxP site, mouse Clstn1 genomic DNA
Research application	Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System
Specific Term and Conditions	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 295(28):9650-9662 (2020). doi: 10.1074/jbc.RA119.012386.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Ace.No. CDB0472K and Web. Site (http ://https://large.riken.jp/distribution/mutant-list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (J. Biol. Chem., 295 9650-9662. doi:10.1074/jbc.RA119.012386). Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
Depositor	Tohru Yamamoto (Kagawa University)
Strain Status	Frozen embryos Frozen sperm
Strain Availability	Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month)
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR-

BRC mice in Publications

No Data