Strain Data Sheet
RBRC11514
Strain Information | |
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| Image | |
| BRC No. | RBRC11514 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6NJcl.Cg-Clstn3<tm1Tymo> |
| Former Common name | Alcadeinβ KO; Calsyntenin-3 KO |
| H-2 Haplotype | |
| ES Cell line | D3 [129S2/SvPas] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Toru Yamamoto, Kagawa University in 2021. This strain was generated using D3 ES cells derived from 129S2/SvPas. Mice were further backcrossed to C57BL/6N for 10 generations. C57BL/6N congenic strain. |
| Strain description | Clstn3 knockout mice. PGK-neo fragment replaced exon 1 to 3 of the Clstn3 gene. |
| Colony maintenance | |
| References | Novel cadherin-related membrane proteins, Alcadeins, enhance the X11-like protein-mediated stabilization of amyloid beta-protein precursor metabolism. Araki Y, Tomita S, Yamaguchi H, Miyagi N, Sumioka A, Kirino Y, Suzuki T J. Biol. Chem., 278(49):49448-58 (2003). 12972431The novel cargo Alcadein induces vesicle association of kinesin-1 motor components and activates axonal transport. Araki Y, Kawano T, Taru H, Saito Y, Wada S, Miyamoto K, Kobayashi H, Ishikawa H O, Ohsugi Y, Yamamoto T, Matsuno K, Kinjo M, Suzuki T EMBO J., 26(6):1475-86 (2007). 17332754A small peptide sequence is sufficient for initiating kinesin-1 activation through part of TPR region of KLC1. Kawano T, Araseki M, Araki Y, Kinjo M, Yamamoto T, Suzuki T Traffic., 13(6):834-48 (2012). 22404616Amyloidogenic processing of amyloid beta protein precursor (APP) is enhanced in the brains of alcadein alpha-deficient mice. Gotoh N, Saito Y, Hata S, Saito H, Ojima D, Murayama C, Shigeta M, Abe T, Konno D, Matsuzaki F, Suzuki T, Yamamoto T J. Biol. Chem., 295(28):9650-9662 (2020). 32467230 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Clstn3 MGI:2178323 | calsyntenin 3 | 6 | Clstn3<tm1Tymo> | targeted mutation 1, Toru Yamamoto | |||
| neo | neomycin resistance gene (E. coli) | 6 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neo, PGK 3’end, mouse Clstn3 genomic DNA |
| Research application | |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol Chem. 2020 Jul 10;295(28):9650-9662. doi: 10.1074/jbc.RA119.012386. |
| Depositor | Tohru Yamamoto (Kagawa University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |