Strain Data Sheet

RBRC11519

Strain Information

Image
BRC No.RBRC11519
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Ucp1<em1(EGFP)Hohu>
Former Common nameUCP1-GFP KI mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Hideyo Ohuchi, Okayama University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionEGFP knockin mice at the Ucp1 locus. EGFP was inserted at 11 bp downstream of the translation initiation codon of the Ucp1.
Colony maintenance
References
UCP1 expression in the mouse adrenal gland is not upregulated by thermogenic conditions.
Fujita H, Habuta M, Hattori T, Kubota S, Kumon H, Ohuchi H
Biochem Biophys Res Commun., 566:184-189 (2021). 34129966

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ucp1
MGI:98894		uncoupling protein 1 (mitochondrial, proton carrier)8Ucp1<em1(EGFP)Hohu> endonuclease mediated mutation 1, Hideyo Ohuchi

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAマウス UCP1, 人工（オワンクラゲGFP改変体） EGFP, 人工（Thosea asigna virus T2A peptide） T2A
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem Biophys Res Commun. 2021 Aug 20;566:184-189. doi: 10.1016/j.bbrc.2021.06.013.
DepositorHideyo Ohuchi (Okayama University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data