Strain Data Sheet

RBRC11522

Strain Information

Image
BRC No.RBRC11522
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6-Cd19<tm1.1(icre)Yoba>
Former Common nameCD19-iCre
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentDeposited by Yoshihiro Baba, Kyushu University in 2021. C57BL/6 genetic background.
Strain descriptioniCre knockin mice at the Cd19 locus. T2A-iCre was inserted at stop codon. FRT-flanked neo gne was deleted.
Colony maintenance
References
Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells.
Yasuda T, Saito Y, Ono C, Kawata K, Baba A, Baba Y
Sci. Rep., 11(1):5524 (2021). 33750849

Health Report

Examination Date / Room / Rack2025/01/07Room:3-ARack:JSentinel mouse program
2024/10/07Room:3-ARack:JSentinel mouse program
2024/04/08Room:3-ARack:JSentinel mouse program
2024/01/09Room:3-ARack:JSentinel mouse program
2023/10/10Room:3-ARack:JSentinel mouse program
2023/07/12Room:3-ARack:JAdditional tests
2023/07/10Room:3-ARack:JSentinel mouse program
2023/04/10Room:3-ARack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cd19
MGI:88319
CD19 antigen7Cd19<tm1.1(icre)Yoba> targeted mutation 1.1, Yoshihiro Baba
  • immunodeficiency, common variable, 2(MedGEN)

  • immunodeficiency, common variable, 3(MedGEN)
  • Frt yeast FRT (flippase recombination target) site7Frt
    T2A Thosea asigna virus 2A peptide (insect)7T2A
    icre phage P1 codon improved Cre (iCre)7icre

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAThosea asigna virus T2A, P1 phage iCre, Saccharomyces cerevisiae Frt site, mouse Cd19 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Yoshihiro Baba) and enter into a Material Transfer Agreement with Kyushu University. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorYoshihiro Baba (Kyushu University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jan 2023

    BRC mice in Publications

    No Data