Strain Data Sheet
RBRC11524
Strain Information | |
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| Image | |
| BRC No. | RBRC11524 |
| Type | Targeted Mutation and Transgene |
| Species | Mus musculus |
| Strain name | B6.Cg-Klb<tm1Yin> Tg(Alb-Klb/EGFP)5Yin |
| Former Common name | KO bkl x Tg Alb-beta EGFP5 |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Yo-ichi Nabeshima in 2021. C57BL/6 congenic strain. |
| Strain description | Compound mice harboring Klb knockout and a transgene expressing Klb fused with EGFP under the control of Albumin promoter. |
| Colony maintenance | |
| References | Impaired negative feedback suppression of bile acid synthesis in mice lacking betaKlotho. Ito S, Fujimori T, Furuya A, Satoh J, Nabeshima Y, Nabeshima Y J. Clin. Invest., 115(8):2202-8 (2005). 16075061Hepatocyte beta-Klotho regulates lipid homeostasis but not body weight in mice. Kobayashi K, Tanaka T, Okada S, Morimoto Y, Matsumura S, Manio M C, Inoue K, Kimura K, Yagi T, Saito Y, Fushiki T, Inoue H, Matsumoto M, Nabeshima Y FASEB J., 30(2):849-62 (2016). 26514166 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 5 | EGFP | ||||
| Klb MGI:1932466 | klotho beta | 5 | Klb<tm1Yin> | targeted mutation 1, Yo-ichi Nabeshima | |||
| neo | neomycin resistance gene (E. coli) | 5 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | ||||
| Klb MGI:1932466 | klotho beta | UN | Klb | mouse Albumin promoter, rabbit beta-globin intron | |||
| SV40 polyA signal | UN | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse Phosphpglycerate kinase promoter, E. coli Neomycin resistance gene, mouse Albumin promoter, rabbit beta-globin intron, mouse beta-Klotho cDNA, jellyfish Enhanced green fluorescent protein cDNA, SV40 Poly A signal |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Clin. Invest., 115(8):2202-8 (2005). FASEB J., 30(2):849-62 (2016). |
| Depositor | Yoichi Nabeshima (Foundation for Biomedical Research and Innovation at Kobe) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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