Strain Data Sheet

RBRC11524

Strain Information

Image
BRC No.RBRC11524
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Klb<tm1Yin> Tg(Alb-Klb/EGFP)5Yin
Former Common nameKO bkl x Tg Alb-beta EGFP5
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeposited by Yo-ichi Nabeshima in 2021. C57BL/6 congenic strain.
Strain descriptionCompound mice harboring Klb knockout and a transgene expressing Klb fused with EGFP under the control of Albumin promoter.
Colony maintenance
ReferencesJ. Clin. Invest., 115(8):2202-8 (2005). 16075061
FASEB J., 30(2):849-62 (2016). 26514166

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Klbklotho beta5Klb<tm1Yin>targeted mutation 1, Yo-ichi Nabeshima

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)5EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Klbklotho betaUNKlbmouse Albumin promoter, rabbit beta-globin intron

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 Poly A signalUN

Ordering Information

Donor DNAmouse Phosphpglycerate kinase promoter, E. coli Neomycin resistance gene, mouse Albumin promoter, rabbit beta-globin intron, mouse beta-Klotho cDNA, jellyfish Enhanced green fluorescent protein cDNA, SV40 Poly A signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Clin. Invest., 115(8):2202-8 (2005). FASEB J., 30(2):849-62 (2016).
DepositorYoichi Nabeshima (Foundation for Biomedical Research and Innovation at Kobe)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data