Strain Data Sheet

RBRC11525

Strain Information

Image
BRC No.RBRC11525
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Clgn-Dcst2/PA)1Nino Dcst1/Dcst2<tm1Osb>
Former Common nameDCST2-PA TG
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Naokazu Inoue, Fukushima Medical University in 2021. Mixed background.
Strain descriptionCompound mutant harboring Dcst1 knockout and a transgene to express fusion protein of Dcst2 with a PA tag under the control of the Clgn promoter.
Colony maintenance
References
Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion.
Inoue N, Hagihara Y, Wada I
Elife, 10:e66313 (2021). 33871360

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dcst1
MGI:1925022		DC-STAMP domain containing 13Dcst1<tm1Osb> targeted mutation 1, Research Institute for Microbial Diseases, Osaka University
Dcst2
MGI:2685606		DC-STAMP domain containing 23Dcst2<tm1Osb> targeted mutation 1, Research Institute for Microbial Diseases, Osaka University
Dcst2 DC-STAMP domain containing 2UNDcst2 mouse Clgn promoter
PA PA tag (GVAMPGAEDDVV) (synthetic) human PodoplaninUNPA

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse Calmegin promoter, mouse Dcst2 cDNA, PA-tag (Synthetic DNA), rabbit beta-globin polyA signal, yeast FRT (flipase recombination target) sites, Phage P1 loxP sites, human PGK promoter, E. coli Neomycin resistance gene, human PGK poly (A), mouse Dcst1 genomic DNA, mouse Dcst2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorNaokazu Inoue (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data