Strain Information | |
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Image | |
BRC No. | RBRC11525 |
Type | Targeted Mutation and Transgene |
Species | Mus musculus |
Strain name | STOCK Tg(Clgn-Dcst2/PA)1Nino Dcst1/Dcst2<tm1Osb> |
Former Common name | DCST2-PA TG |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Naokazu Inoue, Fukushima Medical University in 2021. Mixed background. |
Strain description | Compound mutant harboring Dcst1 knockout and a transgene to express fusion protein of Dcst2 with a PA tag under the control of the Clgn promoter. |
Colony maintenance | |
References | Elife, 10:e66313 (2021). 33871360 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dcst1DC-STAMP domain containing 13Dcst1<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dcst2DC-STAMP domain containing 23Dcst2<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dcst2DC-STAMP domain containing 2UNDcst2mouse Clgn promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PAPA tag (GVAMPGAEDDVV) (synthetic) human PodoplaninUNPA |
Ordering Information | |
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Donor DNA | mouse Calmegin promoter, mouse Dcst2 cDNA, PA-tag (Synthetic DNA), rabbit beta-globin polyA signal, yeast FRT (flipase recombination target) sites, Phage P1 loxP sites, human PGK promoter, E. coli Neomycin resistance gene, human PGK poly (A), mouse Dcst1 genomic DNA, mouse Dcst2 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Naokazu Inoue (Fukushima Medical University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |