Strain Data Sheet

RBRC11531

Strain Information

Image
BRC No.RBRC11531
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Shc2<tm1Paw>
Former Common nameShcB-KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeposited by Sho Kakizawa, Kyoto University in 2021. This strain was originally dveloped by Ryuichi Sakai, Kitasato University. Mice were generated using R1 ES cells derived from 129. This strain has been maintained at mixed genetic background.
Strain descriptionShc2 knockout mice. IRES lacZ and neo cassette were inserted at the exon5 of the Shc2 gene locus.
Colony maintenance
ReferencesSci. Rep., 10, 14475 (2020). 32879382
Neuron, 28(3):819-33 (2000). 11163269

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Shc2SHC (Src homology 2 domain containing) transforming protein 210Shc2<tm1Paw>targeted mutation 1, Tony Pawson

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)10

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)10

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAinternal ribosomal entry site (EMCV), E. coli LacZ gene, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, Mouse ShcB exon 3,4,5 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 28(3):819-33 (2000).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorSho Kakizawa (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data