Strain Data Sheet
RBRC11531
Strain Information | |
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| Image | |
| BRC No. | RBRC11531 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | STOCK Shc2<tm1Paw> |
| Former Common name | ShcB-KO |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Sho Kakizawa, Kyoto University in 2021. This strain was originally dveloped by Ryuichi Sakai, Kitasato University. Mice were generated using R1 ES cells derived from 129. This strain has been maintained at mixed genetic background. |
| Strain description | Shc2 knockout mice. IRES lacZ and neo cassette were inserted at the exon5 of the Shc2 gene locus. |
| Colony maintenance | |
| References | The mammalian ShcB and ShcC phosphotyrosine docking proteins function in the maturation of sensory and sympathetic neurons. Sakai R, Henderson J T, O'Bryan J P, Elia A J, Saxton T M, Pawson T Neuron, 28(3):819-33 (2000). 11163269Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells. Kakizawa S, Kishimoto Y, Yamamoto S, Onga K, Yasuda K, Miyamoto Y, Watanabe M, Sakai R, Mori N Sci. Rep., 10, 14475 (2020). 32879382 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Ires | internal ribosomal entry site (EMCV) | 10 | |||||
| Shc2 MGI:106180 | SHC (Src homology 2 domain containing) transforming protein 2 | 10 | Shc2<tm1Paw> MGI:1933344 | targeted mutation 1, Tony Pawson | |||
| lacZ | beta-galactosidase (E. coli) | 10 | |||||
| neo | neomycin resistance gene (E. coli) | 10 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | internal ribosomal entry site (EMCV), E. coli LacZ gene, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, Mouse ShcB exon 3,4,5 genomic DNA |
| Research application | Fluorescent Proteins/lacZ System Neurobiology Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 28(3):819-33 (2000).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Depositor | Sho Kakizawa (Kyoto University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |