Strain Information | |
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Image | |
BRC No. | RBRC11531 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | STOCK Shc2<tm1Paw> |
Former Common name | ShcB-KO |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Deposited by Sho Kakizawa, Kyoto University in 2021. This strain was originally dveloped by Ryuichi Sakai, Kitasato University. Mice were generated using R1 ES cells derived from 129. This strain has been maintained at mixed genetic background. |
Strain description | Shc2 knockout mice. IRES lacZ and neo cassette were inserted at the exon5 of the Shc2 gene locus. |
Colony maintenance | |
References | Sci. Rep., 10, 14475 (2020). 32879382 Neuron, 28(3):819-33 (2000). 11163269 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Shc2SHC (Src homology 2 domain containing) transforming protein 210Shc2<tm1Paw>targeted mutation 1, Tony Pawson Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)10 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)10 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | internal ribosomal entry site (EMCV), E. coli LacZ gene, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, Mouse ShcB exon 3,4,5 genomic DNA |
Research application | Fluorescent Proteins/lacZ System Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 28(3):819-33 (2000).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Sho Kakizawa (Kyoto University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |