Strain Information | |
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Image | |
BRC No. | RBRC11535 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | B6;129B6-Tg(CAG-Su9/tdTomato/T2A/Puro)1GAitn |
Former Common name | Y-mtRFP |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Ayako Isotani, Nara Institute of Science and Technology in 2021. This strain was generated using mF1-05 ES cells derived from (129X1 x C57BL/6J)F1. Mixed genetic background. |
Strain description | Reporter mice indicating the presence of Y chromosome. CAG-Su9-tdTomato-T2A-Puror is integrated at the Ddx3y locus. |
Colony maintenance | |
References | Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage. Hirata W, Tomoda T, Yuri S, Isotani A Exp. Anim., 71(1):82-89 (2022). 34544911 |
Health Report | |
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Examination Date / Room / Rack | 2025/01/07Room:3-BRack:BSentinel mouse program 2024/10/07Room:3-BRack:BSentinel mouse program 2024/04/08Room:3-BRack:BSentinel mouse program 2024/01/09Room:3-BRack:BSentinel mouse program 2023/10/10Room:3-BRack:BSentinel mouse program |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Su9 | mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)] | Y | Su9 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
T2A | Thosea asigna virus 2A peptide (insect) | Y | T2A | ||||
puro | puromycin acyltransferase (Streptomyces alboniger) | Y | puro | ||||
tdTomato | tandem dimeric Red Fluorescent Protein (Discosoma sp.) | Y | tdTomato |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | CAG promoter (Cytomegalovirus CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Discosoma sp. Red Fluorecent Protein cDNA (tdTomato), Mouse Atp5g1 gene mitochondria localization signal(su9), Thosea asigna ribosomal skip site (T2A), Streptomyces alboniger Puromycin resistant gene |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 71(1):82-89 (2022). For use of the BIOLOGICAL RESOURCE by a for-profit institution and an academic institution for the purpose of profits, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Ayako Isotani (Nara Institute of Science and Technology) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jul 2023 |
BRC mice in Publications |
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No Data |